Hemizygous interstitial deletion of chromosome 15 (band D) in three translocation-negative murine plasmacytomas.
Hemizygous interstitial deletion of chromosome 15 (band D) in three translocation-negative murine plasmacytomas.
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三个易位阴性小鼠浆细胞瘤中 15 号染色体（带 D）的半合子间质缺失。
DOI：
10.1073/pnas.81.4.1159
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发表时间：
1984
影响因子：
11.1
通讯作者：
Potter,M
中科院分区：
文献类型：
--
作者：
Wiener,F;Ohno,S;Babonits,M;Sümegi,J;Wirschubsky,Z;Klein,G;Mushinski,JF;Potter,M
应用G显带和高分辨显带技术，对3例缺乏(12；15)或(6；15)易位特征的小鼠浆细胞瘤进行了研究。在所有三种肿瘤中，每两条15号染色体中就有一条(四倍体肿瘤中四条染色体中有两条)缩短。高分辨显带分析表明，这是由于15D带区的间隙缺失所致。导致缺失的两个断裂已暂时定位于带D2/3‘的界面和带D2内。三种浆细胞瘤中的一种ABPC45含有重排的c-myc基因。这三种肿瘤都含有比正常脾或胸腺更丰富的2.4千碱基myc RNA转录本。C-myc基因位于15D2/3带区域。我们推测它可能加入了缺失的浆细胞瘤的着丝粒部分。这种转位可能导致了它的结构性激活，就像在更频繁的易位携带浆细胞瘤中一样。
Three murine plasmacytomas that were exceptional in lacking the characteristic (12;15) or (6;15) translocations were studied by G banding and high-resolution banding. One of every two chromosomes 15 (two of four in tetraploid tumors) was shortened in all three tumors. High-resolution banding analysis revealed that this was due to an interstitial deletion in the 15D band region. The two breaks responsible for the deletion have been tentatively localized to the interface of bands D2/3' and within band D2. One of the three plasmacytomas, ABPC45, had a rearranged c-myc gene. All three tumors contained a greater abundance of 2.4-kilobase myc RNA transcripts than normal spleen or thymus. The c-myc gene is located in the 15 D2/3 band region. We suggest that it may have joined the centromeric portion in the deletion plasmacytomas. This transposition may have led to its constitutive activation, as in the more frequent translocation-carrying plasmacytomas.