Alterations of membrane curvature during influenza virus budding
Alterations of membrane curvature during influenza virus budding
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DOI：
10.1042/bst20140136
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发表时间：
2014-10-01
影响因子：
3.9
通讯作者：
Rossman, Jeremy
中科院分区：
文献类型：
--
作者：
Martyna, Agnieszka;Rossman, Jeremy
甲型流感病毒属于正粘病毒科。它是一种包膜病毒，含有分段和负义RNA基因组。甲型流感病毒引起每年的流行病和偶尔的大流行病，是世界范围内发病率和死亡率的主要原因，并对社会产生重大的经济影响。新病毒颗粒的组装和出芽是一个复杂的多步骤过程，涉及多种宿主和病毒因子。流感病毒利用极化上皮细胞顶端质膜中的脂筏结构域作为出芽位点。两种病毒糖蛋白，血凝素和神经氨酸酶，集中在脂筏，引起膜曲率的改变和出芽过程的启动。形成病毒粒子内部结构的基质蛋白1（M1）随后被募集到该位点，随后掺入病毒核糖核蛋白和基质蛋白2（M2）。M1可以改变膜的曲率和进展出芽，而脂筏相关的M2稳定出芽的网站，允许病毒粒子的正确组装。在出芽的后期阶段，M2定位于脂质相边界处出芽病毒粒子的颈部，在那里它引起负膜曲率，导致断裂和病毒粒子释放。
Influenza A virus belongs to the Orthomyxoviridae family. It is an enveloped virus that contains a segmented and negative-sense RNA genome. Influenza A viruses cause annual epidemics and occasional major pandemics, are a major cause of morbidity and mortality worldwide, and have a significant financial impact on society. Assembly and budding of new viral particles are a complex and multi-step process involving several host and viral factors. Influenza viruses use lipid raft domains in the apical plasma membrane of polarized epithelial cells as sites of budding. Two viral glycoproteins, haemagglutinin and neuraminidase, concentrate in lipid rafts, causing alterations in membrane curvature and initiation of the budding process. Matrix protein 1 (M1), which forms the inner structure of the virion, is then recruited to the site followed by incorporation of the viral ribonucleoproteins and matrix protein 2 (M2). M1 can alter membrane curvature and progress budding, whereas lipid raft-associated M2 stabilizes the site of budding, allowing for proper assembly of the virion. In the later stages of budding, M2 is localized to the neck of the budding virion at the lipid phase boundary, where it causes negative membrane curvature, leading to scission and virion release.