Plasmodium ovale curtisi and Plasmodium ovale wallikeri in North-West Ethiopia
Plasmodium ovale curtisi and Plasmodium ovale wallikeri in North-West Ethiopia
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DOI：
10.1186/1475-2875-12-346
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发表时间：
2013-09-28
期刊：
影响因子：
3
通讯作者：
Noedl, Harald
中科院分区：
文献类型：
--
作者：
Alemu, Abebe;Fuehrer, Hans-Peter;Noedl, Harald
背景资料：在埃塞俄比亚，恶性疟原虫和间日疟原虫是占优势的物种，分别约占疟疾病例的60%和40%。最近，在全国各地观察到恶性疟原虫向间日疟原虫的重大转变，但其他人类疟疾物种卵形疟原虫的流行病学研究表明，对三日疟原虫的了解仍然很少。本研究的目的是评估卵圆线虫curtisi和wallikeri感染在埃塞俄比亚西北部通过使用显微镜和巢式PCR.Methods：一个卫生机构为基础的调查，使用非概率抽样技术进行了Maksegnet，Enfranze和科拉迪巴卫生中心和Metema医院在北贡达尔。本研究纳入了300名具有与疟疾一致的体征和症状的患者，并收集了毛细血管血用于显微镜检查和疟原虫物种的分子分析。在Whatman 903滤纸上采集样品，储存在带有干燥剂的小塑料袋中，并运输至维也纳（奥地利）进行分子分析。结果：在300例受试者（男167例，女133例）中，184例经显微镜检测为疟疾阳性（恶性疟原虫133例，间日疟原虫51例）。通过种特异性PCR检测233种疟原虫（95% CI：72.6-82），大多数为155（66.5%，95%CI：60.2-72.3）为恶性疟原虫，其次为间日疟原虫69（29.6%，95% CI：24.1-35.8）和9份（3.9%，95% CI：2-7.2）样本为卵形假单胞菌阳性。其中7例为瓦氏卵圆线虫，2例为柯氏卵圆线虫。所有样本均未检测出三日疟原虫阳性。在显微镜检查有高（16.3%）的假阴性报告和所有混合感染和卵形疟原虫的情况下，错过或misclassified.Conclusion：这项研究表明，卵形疟原虫疟疾在埃塞俄比亚报告不足，并提供了第一个已知的证据土著卵形疟原虫wallikeri和卵形疟原虫curtisi在埃塞俄比亚的同域分布。因此，迫切需要进一步研究评估稀有物种卵形疟原虫和三日疟原虫的流行情况，以更好地了解物种分布并调整疟疾控制策略。
Background: In Ethiopia Plasmodium falciparum and Plasmodium vivax are the dominant species accounting for roughly 60 and 40% of malaria cases, respectively. Recently a major shift from P. falciparum to P. vivax has been observed in various parts of the country but the epidemiology of the other human malaria species, Plasmodium ovale spp. and Plasmodium malariae remains poorly understood. The aim of this study was to assess P. ovale curtisi and wallikeri infection in north-west Ethiopia by using microscopy and nested PCR.Methods: A health institution-based survey using non-probability sampling techniques was conducted at Maksegnet, Enfranze and Kola Diba health centres and Metema hospital in North Gondar. Three-hundred patients with signs and symptoms consistent with malaria were included in this study and capillary blood was collected for microscopic examination and molecular analysis of Plasmodium species. Samples were collected on Whatman 903 filter papers, stored in small plastic bags with desiccant and transported to Vienna (Austria) for molecular analysis. Data from study participants were entered and analysed by SPSS 20 software.Results: Out of 300 study participants (167 males and 133 females), 184 samples were classified positive for malaria (133 P. falciparum and 51 P. vivax) by microscopy. By species-specific PCR 233 Plasmodium spp (95% CI: 72.6-82) were detected and the majority 155 (66.5%, 95% CI: 60.2-72.3) were P. falciparum followed by P. vivax 69 (29.6%, 95% CI; 24.1-35.8) and 9 (3.9%, 95% CI: 2-7.2) samples were positive for P. ovale. Seven of P. ovale parasites were confirmed as P. ovale wallikeri and two were confirmed as P. ovale curtisi. None of the samples tested positive for P. malariae. During microscopic examination there were high (16.3%) false negative reports and all mixed infections and P. ovale cases were missed or misclassified.Conclusion: This study indicates that P. ovale malaria is under-reported in Ethiopia and provides the first known evidence of the sympatric distribution of indigenous P. ovale wallikeri and P. ovale curtisi in Ethiopia. Therefore, further studies assessing the prevalence of the rare species P. ovale and P. malariae are urgently needed to better understand the species distribution and to adapt malaria control strategies.