Pleomorphic carcinoma of the lung associated with loss of heterozygosity of p53 gene
Pleomorphic carcinoma of the lung associated with loss of heterozygosity of p53 gene
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DOI：
10.1620/tjem.206.181
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发表时间：
2005-06-01
影响因子：
2.2
通讯作者：
Ono, M
中科院分区：
文献类型：
--
作者：
Arita, N;Mikami, Y;Ono, M
我们报告一例 70 岁男性多形性肺癌病例。多形性癌的特征是异质成分，包括上皮和间质恶性肿瘤。在本病例中，肿瘤由明确的鳞状细胞癌和类似于肉瘤过度生长的梭形细胞成分的混合物组成。纺锤体成分不包括以骨、软骨、神经元或肌肉组织的明显分化为特征的异源间充质成分。为了评估梭形细胞成分的分化状态，我们通过免疫组织化学检查了抗原的表达，包括波形蛋白、细胞角蛋白、肌节肌动蛋白、α-平滑肌肌动蛋白、S-100蛋白、CD34、因子VIII和CD68。结果显示，波形蛋白仅在梭形细胞成分中表达，表明间充质谱系处于未成熟状态。此外，该病例的梭形细胞成分的遗传特征是 p53 基因外显子 7 的密码子 234 处杂合性丢失 (LOH)。这种突变会导致氨基酸替换（Tyr 替换为 Cys），此前已证明这种替换会减弱 p53 的功能。本病例可能提示 p53 基因的体细胞改变与多形性癌的组织发生之间的关系。 - 多形性；癌; p53基因；杂合性丢失 (c) 2005 年东北大学医学出版社。
We report a case with pleomorphic carcinoma of the lung in a 70-year-old man. Pleomorphic carcinoma is characterized by a heterogenous composition that includes epithelial and mesechymal malignancies. In the present case, the tumor was composed of a mixture of unequivocal squamous cell carcinoma and spindle cell components resembling sarcomatous overgrowth. The spindle component did not include a heterologous mesenchymal element characterized by overt differetiation for bone, cartilage, neuron or muscle tissue. To evaluate a state of differentiation of the spindle cell component, we immunohistochemically examined expression of the antigens including vimentin, cytokeratin, sarcomeric actin, a-smooth muscle actin, S-100 protein, CD34, Factor VIII, and CD68. The results showed sole expression of vimentin in the spindle cell component, suggesting an immature state of the mesenchymal lineage. Furthermore, the spindle cell component of this case was genetically characterized by loss of heterozygosity (LOH) at a codon 234 of exon 7 of the p53 gene. This mutation causes an amino-acid replacement (Tyr to Cys), which was previously proven to attenuate p53 function. The present case may suggest a relation between somatic alteration of the p53 gene and histogenesis of pleomorphic carcinoma. - Pleomorphic; carcinoma; p53 gene; loss of heterozygosity (c) 2005 Tohoku University Medical Press.