Cytokine expression in the epidural space: a model of noncompressive disc herniation-induced inflammation.
Cytokine expression in the epidural space: a model of noncompressive disc herniation-induced inflammation.
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DOI：
10.1097/brs.0b013e3182604baa
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发表时间：
2013-01-01
期刊：
影响因子：
3
通讯作者：
Yeomans DC
中科院分区：
文献类型：
--
作者：
Cuéllar JM;Borges PM;Cuéllar VG;Yoo A;Scuderi GJ;Yeomans DC
Animal study Development of an animal model for the study of biochemical changes that occur in the epidural space after intervertebral disc herniation. Although strong evidence for an inflammatory component exists, the biochemical processes underlying pain following disc herniation remain unknown. Epidural lavage was performed in 48 rats after L5 dorsal root ganglion (DRG) exposure at baseline and 3, 6, or 24 hours after placement of autologous nucleus pulposus (NP) (N = 15), saline (N = 15), or NP + an interferon-gamma antibody (anti-IFNγ; N = 18) directly onto the DRG. Multiplex assays quantifying interleukin (IL-)-1-α, IL-1β, IL-2, IL-4, IL-6, IL-10, TNFα, IFNγ and GM-CSF were performed. NP (N = 7) was also analyzed for these cytokines by placing NP into saline and measuring the relative concentration. Cytokines measured low at baseline (0–100pg/ml) in all groups. Compared to saline, NP application caused IL-6 elevation, peaking at T=3hr, that was prevented by anti-IFNγ. NP induced elevation of TNFα, peaking at T=24hr and was prevented by anti-IFNγ. IFNγ was elevated after NP at T=3hr and T=24hr. IL-1α was similar after saline versus NP. The concentrations of IL-1β and IL-10 were elevated at T=3hr, 6hr and 24hr in all groups without between-groups difference. The level of IL-4 peaked at T=3hr in the NP group and was different than saline and NP +anti-IFNγ groups but the time effect was insignificant. There was no change for GM-CSF. The concentration of cytokines measured in normal NP was < 2pg/ml for all cytokines except TNFα. In this model of acute non-compressive disc herniation, NP caused the elevation of epidural IL-6, TNFα and IFNγ; all attenuated by IFNγ blockade. IL-1β and IL-10 were both significantly elevated by saline alone and their response was not prevented by IFNγ blockade. This model may prove useful for the study of the biochemical processes by which NP induces inflammation-induced nerve root irritation and radiculopathic pain.
