Attempted mechanical transmission of lumpy skin disease virus by biting insects
Attempted mechanical transmission of lumpy skin disease virus by biting insects
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DOI：
10.1046/j.1365-2915.2003.00445.x
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发表时间：
2003-09-01
影响因子：
1.9
通讯作者：
Mellor, PS
中科院分区：
文献类型：
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作者：
Chihota, CM;Rennie, LF;Mellor, PS
斯氏按蚊和致倦库蚊(双翅目：蚊科)、稳定蝇Stomoxys calcitrans Linneeus(双翅目：蝇科)和叮咬的库蚊(双翅目：Ceratopogonidae)被允许以块状皮肤病(LSD)感染的动物为食，或通过含有块状皮肤病病毒(LSDV)的血粉上的膜取食。然后，这些节肢动物被允许在感染饲料后的不同时间间隔重新吸食易受感染的牛。在取食后立即通过聚合酶链式反应在昆虫中检测到病毒，并以足够高的滴度使病毒能够传播。然而，没有病毒从受感染的动物传播到易受感染的动物。Stephensi、S.calcirans、C.nubeculosus和Cx.观察到了致倦黄粉虱。
The mosquitoes Anopheles stephensi Liston and Culex quinquefasciatus Say (Diptera: Culicidae), the stable fly Stomoxys calcitrans Linnaeus (Diptera: Muscidae) and the biting midge Culicoides nubeculosus Meigen (Diptera: Ceratopogonidae) were allowed to feed on either lumpy skin disease (LSD) infected animals or through a membrane on a bloodmeal containing lumpy skin disease virus (LSDV). These arthropods were then allowed to refeed on susceptible cattle at various intervals after the infective feed. Virus was detected in the insects by polymerase chain reaction immediately after feeding and at sufficiently high titre to enable transmission to occur. However, no transmission of virus from infected to susceptible animals by An. stephensi, S. calcitrans, C. nubeculosus and Cx. quinquefasciatus was observed.