A ZEBRAFISH HOMOLOG OF THE DROSOPHILA NEUROGENIC GENE NOTCH AND ITS PATTERN OF TRANSCRIPTION DURING EARLY EMBRYOGENESIS
A ZEBRAFISH HOMOLOG OF THE DROSOPHILA NEUROGENIC GENE NOTCH AND ITS PATTERN OF TRANSCRIPTION DURING EARLY EMBRYOGENESIS
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DOI：
10.1016/0925-4773(93)90027-u
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发表时间：
1993-10-01
影响因子：
2.6
通讯作者：
CAMPOSORTEGA, JA
中科院分区：
文献类型：
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作者：
BIERKAMP, C;CAMPOSORTEGA, JA
我们在这里描述的一级结构的斑马鱼同源的果蝇神经基因Notch和其模式的mRNA积累在胚胎发育过程中。该基因产生一个8.5 kb的转录本，编码一种推定的跨膜蛋白，与Notch家族成员具有高度的序列相似性，包括36个EGF样重复序列、3个lin-12/Notch重复序列、6个cdc10/SWI6重复序列、OPA重复序列和一个PEST序列。斑马鱼Notch基因的转录受时空调控。高密度的转录本，最有可能是母体来源的，已经可以在2细胞阶段检测到。在原肠胚形成前期，RNA存在于所有细胞中。然而，在原肠胚形成之后，转录物按照快速变化的模式在胚胎的特定区域积累。在这些区域中的一些中，细胞分裂在Notch表达时发生，在其他细胞分化过程中发生。这适用于各种中胚层衍生物，如预期的脊索，并为不同的神经原基，如神经板和脑泡。这种转录本积累模式表明斑马鱼Notch同源物在区域化和细胞多样化过程中的作用。
We describe here the primary structure of a zebrafish homologue of the Drosophila neurogenic gene Notch and its pattern of mRNA accumulation during embryogenesis. The gene produces a 8.5 kb transcript encoding a putative transmembrane protein with a high degree of sequence similarity to members of the Notch family, comprising 36 EGF-like repeats, three lin-12/Notch repeats, six cdc10/SWI6 repeats, OPA repeats and a PEST sequence. Transcription of the zebrafish Notch gene is spatially and temporally regulated. A high density of transcripts, most probably of maternal origin, can already be detected in the 2-cell stage. During pregastrulation stages, RNA is present in all cells. However, following gastrulation, transcripts accumulate in specific regions of the embryo following a rapidly changing pattern. In some of these regions, cell divisions take place at the time of Notch expression, in others processes of cell differentiation. This holds true for various mesodermal derivatives, such as the prospective notochord, and for different neural primordia, such as the neural plate and the brain vesicles. This pattern of transcript accumulation suggests a role for the zebrafish Notch homologue in processes of regionalization and cell diversification.