Isolation of Human Blood Phagocytes by Counterflow Centrifugation Elutriation
Isolation of Human Blood Phagocytes by Counterflow Centrifugation Elutriation
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通过逆流离心淘洗分离人血液吞噬细胞
DOI：
10.1007/978-1-4684-3662-4_4
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发表时间：
1980
影响因子：
3.7
通讯作者：
C. Valeri
中科院分区：
文献类型：
--
作者：
F. Lionetti;S. Hunt;C. Valeri
逆流离心法分离细胞的原理最早由Lindahl(1948)阐述，他推导出了定义粒子在离心场中的位置的方程，该离心场与向心方向上的流体相反。他设计了一台“逆流”离心机，根据酵母的半径和密度以及介质的粘度和密度，在平衡面上浓缩酵母颗粒(Lindahl，1956)。在“逆流”离心机的早期研究中，马血中的嗜酸性白细胞被浓缩，最终的细胞悬浮液中含有20-30%(Lindahl和Lindahl，1955)。McEwen等人取得了重大进展。(1968)设计了用于标准制备离心机的转子。他们的研究主要是为了生产简单的设备，使逆流细胞分离普遍可用。聚苯乙烯微球、酵母菌、植物和血细胞被分解成直径0-20微米的亚群。在第一次分离白细胞时，将红细胞与白细胞之比为700：1的全血浓缩为4：1。在一个白细胞占73%的全血样本中，逆流离心法在分离室中回收了94%。随着后来设计的原型转子，麦克尤恩等人。(1971)实现了从感染疟疾的猴子血液中回收99%的白细胞。
The principle of cell separation by counterflow centrifugation was first enunciated by Lindahl (1948) who derived an equation defining the position of particles in a centrifugal field opposed by fluid flowing in the centripetal direction. He designed a “counterstreaming” centrifuge which concentrated yeast particles in planes of equilibrium dependent upon the radius and density of the yeast and the viscosity and density of the medium (Lindahl, 1956). In an early study with the “counterstreaming” centrifuge eosinophilic leukocytes from horse blood were concentrated and the final cell suspension contained 20–30% (Lindahl and Lindahl, 1955). A significant advance was made by McEwen et al. (1968) who designed rotors for use with a standard preparative centrifuge. Their studies were largely responsible for the production of the simple equipment which makes counterflow cell separation generally available. Polystyrene micro spheres, yeasts, plant, and blood cells were resolved into subpopulations with diameters which ranged from 0–20 µm. In the first attempts to isolate leukocytes, whole blood with a ratio of 700 red cells to 1 leukocyte was concentrated to a ratio of 4:1. In a sample of whole blood in which 73% of the white cells consisted of granulocytes, counterflow centrifugation recovered 94% of them in the separation chambers. With a prototype rotor designed later, McEwen et al. (1971) achieved 99% recovery of leukocytes from malaria-infected monkey blood.