Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system
Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system
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DOI：
10.1016/s1673-8527(08)60114-9
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发表时间：
2009-05-01
影响因子：
5.9
通讯作者：
Chu, Chengcai
中科院分区：
文献类型：
--
作者：
Ma, Yimian;Liu, Luo;Chu, Chengcai
使用具有启动子捕获和激活标记功能的载体pCAS04产生了大约25,000个水稻T-DNA插入突变系。 Southern blot分析显示，大约40%的突变体是单拷贝整合，平均T-DNA插入数为2.28。通过田间广泛的表型分析，获得了相当多的农艺学上重要的突变体。对 4,3 10 初级突变体进行的组织化学 GUS 测定表明，在各种组织中，GUS 染色频率高于以前的报道，尤其是在花中。分离了一些突变体的 T-DNA 侧翼序列，并将 T-DNA 插入位点定位到水稻基因组。侧翼序列分析证明了水稻基因组中右边界和左边界的不同整合模式。与拟南芥和杨树相比，水稻的T-DNA边界连接处存在很大差异。
About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these Mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,3 10 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.