Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of Ehrlichia chaffeensis and Ehrlichia canis
Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of Ehrlichia chaffeensis and Ehrlichia canis
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DOI：
10.1128/cdli.4.6.731-735.1997
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发表时间：
1997-11-01
期刊：
影响因子：
--
通讯作者：
Walker, DH
中科院分区：
文献类型：
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作者：
Chen, SM;Cullman, LC;Walker, DH
为了评价查菲埃立克体（Ehrlichia chaffeensis）各种抗原蛋白抗体检测对诊断人单核细胞嗜性埃立克体病（human monocytotropic Ehrlichiosis）的相对敏感性，采用间接免疫荧光法对27例有抗体的恢复期患者血清进行了Western免疫印迹分析。15显示出与29/28-kDa蛋白和44-至88-kDa范围内的蛋白的反应性，具有该模式的血清样品中的两个仅与91 HE 17菌株的29/28-kDa蛋白反应，并且一个样品仅与阿肯色州菌株的29/28-kDa蛋白反应，表明抗体被菌株特异性表位刺激。总的来说，仅在16名患者的血清中检测到29/28-kDa蛋白的抗体，这表明该蛋白比120-kDa蛋白敏感性低。12名健康献血者的血清样品中有两份具有与120-kDa蛋白反应的抗体;其中一个样品还与犬埃里希体的29/28-kDa蛋白反应，表明可能发生了未识别的埃里希体感染，包括人感染埃里希体。犬。与120-kDa蛋白质的反应性之间的高度相关性，通过Western免疫印迹和重组120-kDa蛋白质的斑点印迹支持该重组抗原在诊断血清学中的潜在用途。
In order to evaluate the relative sensitivity of the detection of antibodies against various antigenic proteins of Ehrlichia chaffeensis for the diagnosis of the emerging infectious disease human monocytotropic ehrlichiosis, Western immunoblotting was performed with 27 serum samples from convalescent patients with antibodies, as demonstrated by indirect immunofluorescence assay, Among 22 patients with antibodies reactive with the 120-kDa protein, 15 showed reactivity with the 29/28-kDa protein(s) and the proteins in the 44- to 88-kDa range, Two of the serum samples with this pattern reacted with the 29/28-kDa protein(s) of only the 91HE17 strain, and one sample reacted with only that of-the Arkansas strain, indicating that the antibodies were stimulated by strain-specific epitopes. Overall, antibodies to the 29/28-kDa protein(s) were detected in only 16 patients' sera, suggesting that this protein is less sensitive than the 120-kDa protein, Two of 12 serum samples from healthy blood donors had antibodies reactive with the 120-kDa protein; one of these samples reacted also with the 29/28-kDa protein(s) of Ehrlichia canis, suggesting that unrecognized ehrlichial infection might have occurred, including human infection with E. canis. A high correlation between reactivity with the 120-kDa protein by Western immunoblotting and the recombinant 120-kDa protein by dot blot supports the potential usefulness of this recombinant antigen in diagnostic serology.