Leishmania braziliensis: partial control of experimental infection by interleukin-12 p40 deficient mice
Leishmania braziliensis: partial control of experimental infection by interleukin-12 p40 deficient mice
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DOI：
10.1590/s0074-02762004000300009
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发表时间：
2004-05-01
期刊：
影响因子：
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通讯作者：
Afonso, Luís Carlos Crocco
中科院分区：
文献类型：
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作者：
Souza-Neto, Sebastião Martins de;Carneiro, Cláudia Martins;Afonso, Luís Carlos Crocco
对利什曼原虫感染的抗性与依赖于白细胞介素12（IL-12）存在的Th 1型应答的发展相关。在这项工作中，这种细胞因子的参与，在响应感染的L。评价了小鼠模型中毒性较小的物种巴西种。我们的结果表明，当干扰素（IFN-γ）缺陷（-/-）小鼠接种L。尽管IL-12 p40-/-小鼠中的感染发生严重的不受控制的病变，但在IL-12 p40-/-小鼠中观察到慢性病变在感染长达12周时仍处于控制之下。IL 12 p40-/-小鼠在其病变中比IFN-γ-/-小鼠具有更少的寄生虫。来自IL-12 p40-/-的淋巴结细胞能够产生低但一致的IFN-γ水平，这表明其参与寄生虫控制。此外，与以往关于L.主要感染的动物中，在用L.巴西。
Resistance to infection by Leishmania major has been associated with the development of a Th1 type response that is dependent on the presence of interleukin 12 (IL-12). In this work the involvement of this cytokine in the response to infection by L. braziliensis, a less virulent species in the mouse model, was evaluated. Our results show that while interferon (IFN-gamma) deficient (-/-) mice inoculated L. braziliensis develop severe uncontrolled lesions, chronic lesions that remained under control up to 12 weeks of infection were observed in IL-12p40 -/- mice. IL 12p40 -/- mice had fewer parasites in their lesions than IFN-gamma-/- mice. Lymph node cells from IL-12p40 -/- were capable of producing low but consistent levels of IFN-gamma suggestive of its involvement in parasite control. Furthermore, as opposed to previous reports on L. major-infected animals, no switch to a Th2 response was observed in IL-12p40 -/- infected with L. braziliensis.