Functional importance of Stat3 tetramerization in activation of the α2-macroglobulin gene
Functional importance of Stat3 tetramerization in activation of the α2-macroglobulin gene
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DOI：
10.1074/jbc.m104978200
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发表时间：
2001-09-07
影响因子：
4.8
通讯作者：
Darnell, JE
中科院分区：
文献类型：
--
作者：
Zhang, XK;Darnell, JE
转染实验发现，四聚体Stat3复合体对白细胞介素-6诱导的α - 2巨球蛋白基因启动子的最大激活至关重要。纯化磷酸化Stat3的稳定四聚体形成依赖于蛋白质。涉及Stat3 n端结构域的蛋白质相互作用。四聚体形成的功能重要性显示在与半形态相关的转录激活水平下降。n端残基突变。
A tetrameric, Stat3 complex was found to be essential in transfection experiments for maximal interleukin-6-inducible activation of alpha2-macroglobulin gene promoter. Stable tetramer formation of purified phosphorylated Stat3 was dependent on protein.protein interaction involving the N-terminal domain of Stat3. The functional importance of tetramer formation was shown by the decreased levels of transcriptional activation associated with hypomorphic. mutations in N-terminal residues.