In vitro stimulation of vitellogenin expression by insulin in the mud crab, Scylla paramamosain, mediated through PI3K/Akt/TOR pathway
In vitro stimulation of vitellogenin expression by insulin in the mud crab, Scylla paramamosain, mediated through PI3K/Akt/TOR pathway
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胰岛素通过 PI3K/Akt/TOR 通路介导体外刺激青蟹中卵黄蛋白原的表达
DOI：
10.1016/j.ygcen.2017.06.013
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发表时间：
2017-09-01
影响因子：
2.7
通讯作者：
Ye, Haihui
中科院分区：
文献类型：
--
作者：
Huang, Xiaoshuai;Feng, Biyun;Ye, Haihui
卵黄蛋白原（vtg）的合成是卵生动物卵巢发育的重要过程之一，又称卵黄发生。最近，由于转录组测序的应用，已经报道了多种胰岛素样肽（ILP）在甲壳动物物种。在这种情况下，本研究报告，除了外源性ILP，牛胰岛素，刺激VTG（称为SP-VTG）的表达在肝胰腺外植体从青蟹，拟穴青蟹，通过体外实验。ILP信号转导关键因子Sp-PI 3 K、Sp-Akt、Sp-Rheb和Sp-TOR的同源基因已在S. paramamosain基于转录组数据库。进一步的实验表明，RNAi介导的Sp-Akt基因敲低以及Sp-PI 3 K和Sp-TOR抑制剂阻断了胰岛素对Sp-vtg表达的刺激。综合结果暗示内源性ILP及其相应的信号转导在S.拟穴龙(C)2017爱思唯尔公司All rights reserved.
Vitellogenin (vtg) synthesis, known as vitellogenesis, is one of most important processes in the ovarian development of oviparous animals. Recently, multiple insulin-like peptides (ILPs) have been reported in crustacean species due to the application of transcriptome sequencing. In this context, the present study reports that the addition of an exogenous ILP, bovine insulin, stimulates vtg (termed Sp-vtg) expression in hepatopancreatic explants from the mud crab, Scylla paramamosain, by in vitro experiments. Homologous genes of key factors in ILP signaling, Sp-PI3K, Sp-Akt, Sp-Rheb and Sp-TOR, have been isolated in S. paramamosain based on a transcriptome database. Further experiments reveal that the RNAi-mediated Sp-Akt gene knockdown and the inhibitors of Sp-PI3K and Sp-TOR block the stimulation of Sp-vtg expression by insulin. The combined results implicate the endogenous ILP and its corresponding signaling in the regulation of Sp-vtg synthesis in S. paramamosain. (C) 2017 Elsevier Inc. All rights reserved.