Gas chromatography-mass spectrometry metabolite profiling of worker honey bee (Apis mellifera L.) hemolymph for the study of Nosema ceranae infection
Gas chromatography-mass spectrometry metabolite profiling of worker honey bee (Apis mellifera L.) hemolymph for the study of Nosema ceranae infection
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DOI：
10.1016/j.jinsphys.2012.07.010
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发表时间：
2012-10-01
影响因子：
2.2
通讯作者：
Jabaji, Suha
中科院分区：
文献类型：
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作者：
Aliferis, Konstantinos A.;Copley, Tanya;Jabaji, Suha
在这里，我们提出了一个气相色谱-质谱（GC/MS）的方法研究感染的工蜂（意大利蜜蜂L。）新出现的专性细胞内寄生虫Nosema cerebrovirus的血淋巴代谢物谱的基础上。由于该病的严重性，早期发现对其有效控制至关重要。结果表明，寄生虫引起蜜蜂的生理学的一般干扰，影响控制受感染个体能量储备动员的机制。对宿主施加的营养和能量应激主要表现为大多数碳水化合物和氨基酸水平的降低，包括代谢物如果糖、L-脯氨酸以及冷冻保护剂山梨醇和甘油，这些物质涉及各种生化途径。有趣的是，在感染的蜜蜂中检测到的葡萄糖水平显著更高。代谢组学分析与多重定量PCR分析一致，表明它可以用作检测和研究疾病生理学的补充工具。(C)2012爱思唯尔有限公司保留所有权利。
Here, we are presenting a gas chromatography-mass spectrometry (GC/MS) approach for the study of infection of the worker honey bee (Apis mellifera L.) by the newly emerged obligate intracellular parasite Nosema ceranae based on metabolite profiling of hemolymph. Because of the severity of the disease, early detection is crucial for its efficient control. Results revealed that the parasite causes a general disturbance of the physiology of the honey bee affecting the mechanisms controlling the mobilization of energy reserves in infected individuals. The imposed nutritional and energetic stress to the host was depicted mainly in the decreased levels of the majority of carbohydrates and amino acids, including metabolites such as fructose, L-proline, and the cryoprotectants sorbitol and glycerol, which are implicated in various biochemical pathways. Interestingly, the level of glucose was detected at significantly higher levels in infected honey bees. Metabolomics analyses were in agreement with those of multiplex quantitative PCR analyses, indicating that it can be used as a complementary tool for the detection and the study of the physiology of the disease. (C) 2012 Elsevier Ltd. All rights reserved.