Analysis of methylation-sensitive amplified polymorphism in different cotton accessions under salt stress based on capillary electrophoresis
Analysis of methylation-sensitive amplified polymorphism in different cotton accessions under salt stress based on capillary electrophoresis
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DOI：
10.1007/s13258-015-0301-6
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发表时间：
2015-06
期刊：
影响因子：
2.1
通讯作者：
Baohua Wang;R. Fu;Mi Zhang;Zhenqian Ding;Lei Chang;Xinyu Zhu;Yafeng Wang;Baoxiang Fan;
中科院分区：
文献类型：
--
作者：
Baohua Wang;R. Fu;Mi Zhang;Zhenqian Ding;Lei Chang;Xinyu Zhu;Yafeng Wang;Baoxiang Fan;
采用毛细管电泳甲基化敏感扩增多态性（methylation-sensitive amplification polymorphism，MSP）方法，对3份棉花材料（耐盐品种中棉所35和中07，盐敏感品种中棉所12）的DNA甲基化水平进行了分析。盐胁迫下，三种棉花基因组DNA均发生了高甲基化、低甲基化等多种类型的甲基化。耐盐材料中棉所35和中07的超甲基化发生率显著高于低甲基化。而盐敏感品种中棉所12的低甲基化发生率显著高于高甲基化。总体而言，盐胁迫下耐盐材料中棉所35和中07的DNA甲基化水平均显著升高，而盐敏感材料中棉所12的DNA甲基化水平差异不显著。结果表明，耐盐棉花可能存在响应盐胁迫而提高甲基化水平的机制; DNA甲基化水平的整体提高以及不同的甲基化模式可能在棉花耐盐性中起重要作用。通过对棉花不同甲基化程度DNA序列的克隆和分析，发现了一些可能与棉花耐盐性相关的基因。
A methylation-sensitive amplification polymorphism method based on capillary electrophoresis was used to analyze DNA methylation levels in three cotton accessions, two salt-tolerant accessions CCRI 35 and Zhong 07 and one salt-sensitive accession CCRI 12. Many categories of DNA methylation happened in the three cotton accessions under salt treatment, including hypermethylation, hypomethylation, and other patterns. Hypermethylation happened at a significantly higher rate than that of hypomethylation in salt-tolerant accessions CCRI 35 and Zhong 07. On the contrary, in salt-sensitive accession CCRI 12, hypomethylation happened at a significantly higher rate than that of hypermethylation. In general, the global DNA methylation level significantly increased under salt stress in both salt-tolerant accessions CCRI 35 and Zhong 07, whereas there was no significant difference in the salt-sensitive accessions CCRI 12. Our results suggested that salt-tolerant cotton might have a mechanism of increasing the methylation level when responding to salt stress; the increase of the global level of DNA methylation and also different methylation patterns might play important roles in tolerance to salt stress in cotton. Some interesting genes were found through cloning and analysis of differently methylated DNA sequences, which might contribute to salt tolerance in cotton.