Upstream Factors Regulating Maturation/M-Phase Promoting Factor Activity During Oocyte Maturation
Upstream Factors Regulating Maturation/M-Phase Promoting Factor Activity During Oocyte Maturation
复制标题
DOI：
10.1274/jmor.27.27
复制
发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
K. Naito;Yukio Nishimura;T. Yamamuro;Takuma Shimaoka;W. Fujii;Mari Suzuki;T. Nishimura;K. Kano
中科院分区：
文献类型：
--
作者：
K. Naito;Yukio Nishimura;T. Yamamuro;Takuma Shimaoka;W. Fujii;Mari Suzuki;T. Nishimura;K. Kano
摘要：成熟/M期促进因子(MPF)活性受Cyclin B浓度和CDc2激酶磷酸化状态的调节。本文综述了细胞周期蛋白B的合成和降解以及细胞周期蛋白2的磷酸化和去磷酸化的调控因素。我们主要研究了Aurora A和胞浆多聚腺苷化元件结合蛋白(CPEB)对细胞周期蛋白B合成的影响；后期促进复合体(APC)激活因子FZR1和CDC20对细胞周期蛋白B的降解作用；CDk7和Wee1B对细胞周期蛋白2的磷酸化作用；CDC25对细胞周期蛋白2的去磷酸化作用。我们描述了我们在猪卵母细胞中关于这些因素的最新结果，并讨论了猪卵母细胞成熟过程中MPF活性的整体调节。
Abstract: Maturation/M-phase promoting factor (MPF) activity is regulated by the Cyclin B concentration and phosphorylation states of Cdc2 kinase. In the present review, we summarize the factors regulating the synthesis and degradation of Cyclin B and the phosphorylation and dephosphorylation of Cdc2. We focuse on Aurora A and cytoplasmic polyadenylation element binding protein (CPEB), which are involved in cytoplasmic polyadenylation, for Cyclin B synthesis; on anaphase-promoting complex (APC) activating factors, FZR1 and CDC20, for Cyclin B degradation; on Cdk7 and Wee1B for Cdc2 phosphorylation, and on Cdc25 for Cdc2 dephosphorylation. We describe our recent results concerning these factors in porcine oocytes, and discusse overall regulation of MPF activity during porcine oocyte maturation.