Characterization of a CTX-M-15 Producing Klebsiella Pneumoniae Outbreak Strain Assigned to a Novel Sequence Type (1427).
Characterization of a CTX-M-15 Producing Klebsiella Pneumoniae Outbreak Strain Assigned to a Novel Sequence Type (1427).
复制标题
DOI：
10.3389/fmicb.2015.01250
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Friedrich AW
中科院分区：
文献类型：
--
作者：
Zhou K;Lokate M;Deurenberg RH;Arends J;Lo-Ten Foe J;Grundmann H;Rossen JW;Friedrich AW
产广谱内酰胺酶肺炎克雷伯菌已成为主要的医院病原菌之一。2012年7月至9月期间，一种产生肺炎克雷伯菌的CTX-M-15在荷兰一家大学医院引发了疫情。暴发分离株被鉴定为一种新的序列型（ST1427）。患者接触追踪和全基因组系统发育分析支持了受影响患者之间的流行病学联系。从指标患者不同身体部位获得的多个分离株中检测到菌株内多态性，这可能与抗生素治疗和/或宿主适应有关。在病房甚至在医疗设备上都发现了由疫情克隆引起的环境污染。新克隆与任何已知的地方性/流行病克隆没有密切关系，但携带一组质粒携带的抗性基因[blaCTX−M−15，blaTEM−1,blaOXA−1,aac(6 ')-Ib-cr, qnrB1, tetA(a), aac(3)-II]。对其毒力因子的分析发现了一个以前未被发现的荚膜生物合成区和两个未被发现的菌毛基因簇，表明新克隆不具有高毒力。据我们所知，这是肺炎克雷伯菌ST1427的首次暴发报告，我们的研究可能有助于了解这一新出现的克隆的特征。
Extended-spectrum -lactamase producing Klebsiella pneumoniae have emerged as one of the major nosocomial pathogens. Between July and September 2012, a CTX-M-15 producing K. pneumoniae caused an outbreak in a university hospital in the Netherlands. The outbreak isolates were characterized and assigned to a novel sequence type (ST1427). An epidemiological link between affected patients was supported by patient contact tracing and whole-genome phylogenetic analysis. Intra-strain polymorphism was detected among multiple isolates obtained from different body sites of the index patient, which may relate to antibiotic treatment and/or host adaptation. Environmental contamination caused by the outbreak clone was found in the patient rooms even on medical equipment. The novel clone was not closely related to any known endemic/epidemic clone, but carried a set of a plasmid-borne resistance genes [blaCTX−M−15, blaTEM−1, blaOXA−1, aac(6′)-Ib-cr, qnrB1, tetA(A), aac(3)-II]. Analysis of its virulence factors revealed a previously uncharacterized capsular biosynthesis region and two uncharacterized fimbriae gene clusters, and suggested that the new clone was not hypervirulent. To our knowledge, this is the first outbreak report of K. pneumoniae ST1427, and our study could be of help to understand the features of this newly emerging clone.