Poly(L-lysine)-g-poly(D,L-lactic-co-glycolic acid) micelles for low cytotoxic biodegradable gene delivery carriers
Poly(L-lysine)-g-poly(D,L-lactic-co-glycolic acid) micelles for low cytotoxic biodegradable gene delivery carriers
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DOI：
10.1016/s0168-3659(02)00131-1
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发表时间：
2002-07-18
影响因子：
10.8
通讯作者：
Park, TG
中科院分区：
文献类型：
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作者：
Jeong, JH;Park, TG
合成了聚乳酸-羟基乙酸共聚物（PLGA）接枝聚赖氨酸（PLL）（PLL-g-PLGA），以证明其在水溶液中的胶束形成性能。胶束被用作基因递送载体。PLL-g-PLGA胶束在水溶液中的流体动力学直径约为1.5 μ m。149 nm，尺寸分布窄。临界胶束浓度（cmc）为9.6 mg/l。PLL-g-PLGA胶束可与质粒DNA形成纳米复合物，有效地保护复合DNA不被DNase I降解。胶束/DNA复合物具有高度致密的结构，尺寸在200-300 nm之间，具有正表面电荷值。PLL-g-PLGA胶束具有比PLL更高的转染效率和更低的细胞毒性。本研究表明，可生物降解的阳离子PLL-g-PLGA胶束可作为一种有效的DNA缩合载体用于基因传递系统。(C)2002 Elsevier Science B. V.保留所有权利。
Poly(lactic-co-glycolic acid) (PLGA)-grafted poly(L-lysine) (PLL) (PLL-g-PLGA) was synthesized to demonstrate its micelle-forming property in an aqueous solution. The micelles were used as a gene delivery carrier. The hydrodynamic diameter of PLL-g-PLGA micelles in an aqueous solution was ca. 149 nm with a narrow size distribution. Critical micelle concentration (cmc) was 9.6 mg/l. The PLL-g-PLGA micelles could be used to produce compact nanoparticulate complexes with plasmid DNA, which could efficiently protect the complexed DNA from enzymatic degradation by DNase I. The micelle/DNA complexes had highly compacted structure sized between 200-300 nm with a positive surface charge value. The PLL-g-PLGA micelles exhibited much higher transfection efficiency with lower cytotoxicity than PLL. Here, we demonstrated that biodegradable and cationic PLL-g-PLGA micelles could be used as an effective DNA condensation carrier for gene delivery system. (C) 2002 Elsevier Science B.V. All rights reserved.