Comparison of mice born after intracytoplasmic sperm injection with in vitro fertilization and natural mating.
Comparison of mice born after intracytoplasmic sperm injection with in vitro fertilization and natural mating.
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胞浆内单精子注射后出生的小鼠与体外受精和自然交配的比较。
DOI：
10.1002/mrd.20644
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发表时间：
2007
影响因子：
2.5
通讯作者：
Trounson,Alan
中科院分区：
文献类型：
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作者：
Wilson,TrevorJ;Lacham-Kaplan,Orly;Gould,Jodee;Holloway,Andrew;Bertoncello,Ivan;Hertzog,PaulJ;Trounson,Alan
体外受精（IVF）和卵胞浆内单精子注射（ICSI）的程序通常用于现代医学，以克服不育症，并在畜牧业中，繁殖生育力受损的品系。然而，人工选择和将整个精子注射到卵母细胞中可能会导致出生前和出生后的发育缺陷。为了解决这个问题，我们使用基因表达谱和免疫表型来表征这些程序产生的后代。我们使用谷胱甘肽过氧化物酶1敲除（Gpx 1 −/−）小鼠的配子作为对人工生殖技术（ART）氧化应激反应的致敏筛选。IVF和ICSI衍生后代的基因表达模式没有差异，造血参数略有差异。此外，这些IVF和ICSI幼仔与自然交配的幼仔之间只有微小差异。这些数据首次证明，与IVF相比，ICSI没有显著的表型影响，并且与自然交配相比，我们发现人工受精方法对后代表型的影响相对较小。这些观察结果将支持使用ICSI的突变小鼠系的衍生，并可能在人类ART. Mol. Reprod. Dev. 74：512-519，2007.© 2006 Wiley利斯公司
The procedures of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are routinely used in modern medicine to overcome infertility and, in animal husbandry, to propagate lines with compromised fertility. However, there remains concern that manual selection and injection of whole sperm into oocytes could contribute to pre‐ and postnatal developmental defects. To address this, we have used gene expression profiling and immunophenotyping to characterize offspring generated by these procedures. We used gametes from glutathione peroxidase 1 knockout (Gpx1−/−) mice as a sensitized screen responsive to oxidative stress from artificial reproduction technologies (ART). There were no differences between IVF and ICSI derived offspring in gene expression patterns, and minor differences in hematopoietic parameters. Furthermore there were only minor differences between these IVF and ICSI pups and those derived from natural mating. These data demonstrate for the first time in that there is no significant phenotypic affects of ICSI when compared to IVF and we identified a relatively minor influence of the artificial fertilization methods on phenotype of offspring compared with natural mating. These observations would support the use of ICSI for derivation of mutant mouse lines and may be of some importance for the use of this technique in human ART. Mol. Reprod. Dev. 74: 512–519, 2007. © 2006 Wiley‐Liss, Inc.