Rearrangement at the 5' end of amplified c-myc in human COLO 320 cells is associated with abnormal transcription
Rearrangement at the 5' end of amplified c-myc in human COLO 320 cells is associated with abnormal transcription
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人 COLO 320 细胞中扩增的 c-myc 5 端重排与异常转录相关
DOI：
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发表时间：
1986
影响因子：
5.3
通讯作者：
M. Bishop
中科院分区：
文献类型：
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作者：
M. Schwab;K. Klempnauer;K. Alitalo;H. Varmus;M. Bishop
原癌基因 c-myc 在携带均匀染色染色体区域或双分钟的人 COLO 320 细胞亚系中扩增。携带均匀染色染色体区域的 COLO 320 细胞具有 15 至 20 个明显正常的 c-myc 等位基因拷贝和 1 至 2 个缺乏外显子 1 的异常 c-myc 等位基因拷贝，并表达高水平的正常 c-myc mRNA，大小为 2.5 KB。携带双分钟的 COLO 320 细胞的正常等位基因和异常等位基因各约 25 个拷贝，但优先表达大小为 2.2 KB 的异常 c-myc mRNA。核苷酸序列分析表明，导致异常等位基因中外显子 1 丢失的重排断点位于人和鼠 B 细胞肿瘤中频繁重排的区域内。
The proto-oncogene c-myc is amplified in sublines of human COLO 320 cells carrying either homogeneously staining chromosomal regions or double minutes. COLO 320 cells carrying homogeneously staining chromosomal regions have 15 to 20 copies of an apparently normal c-myc allele and 1 to 2 copies of an abnormal c-myc allele lacking exon 1 and express high levels of a normal c-myc mRNA 2.5 kilobases in size. COLO 320 cells carrying double minutes have about 25 copies each of the normal allele and the abnormal allele but express preferentially an abnormal c-myc mRNA 2.2 kilobases in size. Nucleotide sequence analyses revealed that the break point of rearrangement resulting in the loss of exon 1 in the abnormal allele lies within a region frequently rearranged in human and murine B-cell tumors.
DOI：
10.1073/pnas.81.24.7742
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
RAMSAY, G;EVAN, GI;BISHOP, JM
通讯作者：
BISHOP, JM