Single-Cell Printer: Automated, On Demand, and Label Free
Single-Cell Printer: Automated, On Demand, and Label Free
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DOI：
10.1177/2211068213497204
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发表时间：
2013-12-01
期刊：
影响因子：
--
通讯作者：
Koltay, Peter
中科院分区：
文献类型：
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作者：
Gross, Andre;Schoendube, Jonas;Koltay, Peter
在过去的几年中，单细胞分析已经发展成为细胞生物学中的一个关键主题，以研究无法通过研究更大的细胞群体获得的细胞功能。旨在访问单细胞以在单细胞水平上提取有关其生理学、表型和基因型的信息的工程方法正在以多种方式进行，同时允许分离、分选、培养和分析单个细胞。基于我们早期的研究对喷墨打印单细胞，本文提出了进一步的表征结果与一个完全自动化的原型仪器打印的单活细胞在非接触式喷墨的方式。所提出的技术是基于一个透明的微流控滴按需分配器芯片与相机辅助自动检测系统相结合。芯片内的细胞在从喷嘴排出之前被检测并分类，从而实现每个微滴一个细胞的打印模式。为了证明原型仪器的生物和生物医学应用的适用性，基本的实验，如打印的单珠和细胞阵列，以及沉积和培养的单细胞在微孔板。印刷效率大于80%，可行率约90%。
Within the past years, single-cell analysis has developed into a key topic in cell biology to study cellular functions that are not accessible by investigation of larger cell populations. Engineering approaches aiming to access single cells to extract information about their physiology, phenotype, and genotype at the single-cell level are going manifold ways, meanwhile allowing separation, sorting, culturing, and analysis of individual cells. Based on our earlier research toward inkjet-like printing of single cells, this article presents further characterization results obtained with a fully automated prototype instrument for printing of single living cells in a noncontact inkjet-like manner. The presented technology is based on a transparent microfluidic drop-on-demand dispenser chip coupled with a camera-assisted automatic detection system. Cells inside the chip are detected and classified with this detection system before they are expelled from the nozzle confined in microdroplets, thus enabling a one cell per droplet printing mode. To demonstrate the prototype instrument's suitability for biological and biomedical applications, basic experiments such as printing of single-bead and cell arrays as well as deposition and culture of single cells in microwell plates are presented. Printing efficiencies greater than 80% and viability rates about 90% were achieved.