Dendritic changes in the hippocampal formation of AIDS patients:: a quantitative Golgi study
Dendritic changes in the hippocampal formation of AIDS patients:: a quantitative Golgi study
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DOI：
10.1007/s00401-003-0781-3
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发表时间：
2004-02-01
影响因子：
12.7
通讯作者：
Paula-Barbosa, MM
中科院分区：
文献类型：
--
作者：
Sá, MJ;Madeira, MD;Paula-Barbosa, MM
我们先前已经证明，在获得性免疫缺陷综合征（AIDS）患者的海马结构中，存在神经元萎缩，但没有细胞丢失。由于神经元大小的减少提示相关的神经炎性改变，我们决定研究海马结构中主要神经元群体的树突树。材料获得了5名男性艾滋病患者和5名男性对照。高尔基体浸渍后，用手描记齿状颗粒和门篮细胞以及CA 3和CA 1锥体细胞的树突分支，并对其节段进行分类、计数和测量。我们发现在AIDS组的所有神经元群体中树突树的扩张，这在门和CA 3区更引人注目。具体而言，门神经元有较少的树突节段，并减少分支密度和树突的程度;在CA 3金字塔有一个减少的数量在基底树的终末节段，和减少的总数量的节段，中阶终端的数量，树突的分支密度和树突的程度在顶端树。在CA 1锥体，终端较短的顶端树和树突棘密度下降的基础树，而在颗粒细胞中，只有树突棘密度减少艾滋病患者。观察到提示树突重组的细微迹象。这些结果表明，该区域的脆弱性的海马结构的HIV感染，并可能有助于解释痴呆症的发生，作为海马神经元的接受表面的整体减少的结果。
We have previously shown that in the hippocampal formation of patients with acquired immunodeficiency syndrome (AIDS) there is neuronal atrophy, without cell loss. Because reductions in neuronal size are suggestive of associated neuritic alterations, we decided to study the dendritic trees of the main neuronal populations in the hippocampal formation. Material was obtained in five male AIDS patients and five male controls. After Golgi impregnation, the dendritic arborizations of dentate granule and hilar basket cells, and of CA3 and CA1 pyramidal cells, were hand traced, and their segments classified, counted and measured. We found an impoverishment of the dendritic trees in all neuronal populations in the AIDS group, which was more striking in the hilus and CA3 field. Specifically, hilar neurons had fewer dendritic segments, and reduced branching density and dendritic extent; in CA3 pyramids there was a decrease in the number of terminal segments in the basal trees, and a reduction in the total number of segments, number of medium order terminals, dendritic branching density and dendritic extent in the apical trees. In CA1 pyramids, the terminals were shorter in the apical trees and the dendritic spine density decreased in the basal trees, whereas in granule cells only the dendritic spine density was reduced in AIDS patients. Subtle signs suggestive of dendritic reorganization were observed. These results point to a regional vulnerability of the hippocampal formation to HIV infection, and might contribute to explaining the occurrence of dementia, as a consequence of overall reduction in the hippocampal neuronal receptive surface.