HOMEOSTATIC REGULATION OF CELLULAR ENERGY-METABOLISM - EXPERIMENTAL CHARACTERIZATION INVIVO AND FIT TO A MODEL
HOMEOSTATIC REGULATION OF CELLULAR ENERGY-METABOLISM - EXPERIMENTAL CHARACTERIZATION INVIVO AND FIT TO A MODEL
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DOI：
10.1152/ajpcell.1978.234.3.c82
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发表时间：
1978-01-01
影响因子：
--
通讯作者：
NISHIKI, K
中科院分区：
文献类型：
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作者：
ERECINSKA, M;WILSON, DF;NISHIKI, K
对分离的肝细胞、培养的肾细胞、原生动物(四膜虫)和酵母(念珠菌)的测量表明，细胞能量代谢的动态平衡调节是共同起源的。在每种情况下，从线粒体内的NAD对到细胞色素c的还原等价物的转移和胞质中的ADP到ATP的磷酸化之间几乎保持平衡。在能量需求恒定的条件下，细胞内磷酸盐浓度的变化引起[ATP]/[ADP]的调节，以维持恒定的[ATP]/[ADP][PI]和恒定的呼吸速率。线粒体呼吸的调节是还原的细胞色素c被分子氧氧化的反应的一部分。在[ATP]/[ADP][PI]相近的情况下，呼吸频率随着细胞色素c的减少而增加。线粒体呼吸控制模型被发现与所有不同类型受试细胞的数据很好地吻合。
Measurements in isolated liver cells, cultured kidney cells, protozoa (Tetrahymena pyriformis), and yeast (Candida utilis) indicate that homeostatic regulation of cellular energy metabolism is of common origin. In every case near equilibrium is maintained between the transfer of reducing equivalents from the intramitochondrial NAD couple to cytochrome c and the phosphorylation of cytosolic ADP to ATP. Under conditions of constant energy demand, changes in the intracellular phosphate concentration cause an adjustment in the [ATP]/[ADP] to maintain a constant [ATP]/[ADP][Pi] and constant respiratory rate. The regulation of mitochondrial respiration occurs as part of the reactions by which reduced cytochrome c is oxidized by molecular oxygen. At similar values for the [ATP]/[ADP][Pi] the respiratory rate increases with increasing reduction of cytochrome c. A model for mitochondrial respiratory control is found to give a good fit to the data in all of the different types of cells tested.