Relationship of substance P to afferent characteristics of dorsal root ganglion neurones in guinea-pig
Relationship of substance P to afferent characteristics of dorsal root ganglion neurones in guinea-pig
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DOI：
10.1111/j.1469-7793.1997.00177.x
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发表时间：
1997-11-15
影响因子：
5.5
通讯作者：
Perl, ER
中科院分区：
文献类型：
--
作者：
Lawson, SN;Crepps, BA;Perl, ER
1.在麻醉豚鼠上观察了L_6和S_1背根神经节（DRG）神经元胞体的传入特性与P物质样免疫反应（SP-LI）的关系。玻璃移液管微电极填充荧光染料用于进行细胞内记录和标记DRG胞体。背根传导速度（CV）和每个单位的传入接受特性进行了分类，根据其他物种建立的标准。类别包括各种低阈值机械感受类，无害的热感受类和几个伤害性类。根据CV和感受野的位置（浅表皮肤、深部皮肤或皮下）进一步细分伤害性感受单位. SP-LI用亲和素-生物素复合物法测定，相对染色强度用图像分析法测定。标记强度的可能意义进行了讨论。117个神经元中有29个出现清晰的SP-LI。所有SP-LI阳性单位与确定的感受性伤害性，但不是所有类别的伤害性感受器是积极的。SP-LI标记的强度变化，往往是系统的，在传入特性。CV较慢和/或胞体较小的伤害性神经元有SP-LI阳性的倾向。51个C纤维神经元中19个呈SP样阳性。不到一半的C多模态伤害感受器（CPM）是阳性的。最强烈的标记单位是深皮肤伤害感受器和一些无毛皮肤中的CPM。C低阈值机械感受器和冷敏感单位均未显示SP-LI。66个A纤维神经元中有10个表现出SP-LI，包括16个AS伤害感受器中的8个和15个A α/β伤害感受器中的2个。显示SP-LI的纤维神经元包括七个深皮机械伤害感受器和一些浅皮机械热伤害感受器的多毛皮肤。相反，20个表层皮肤A高阈值机械感受器单位或35个A纤维低阈值单位（D毛和其他单位）均未检测到SP-LI。我们的结论是，豚鼠DRG神经元的SP-LI标记与（a）传入接受特性，（B）外周接受终末所在的组织，（c）CV和（d）索马大小有关。
1. The relationship between the afferent properties and substance P-like immunoreactivity (SP-LI) of L6 and S1 dorsal root ganglion (DRG) neuronal somata was examined in anaesthetized guinea-pigs. Glass pipette microelectrodes filled with fluorescent dyes were used to make intracellular recordings and to label DRG somata. The dorsal root conduction velocity (CV) and the afferent receptive properties of each unit were categorized according to criteria established in other species. Categories included a variety of low threshold mechanoreceptive classes, innocuous thermoreceptive and several nociceptive classes. Nociceptive units were further subdivided on the basis of CV and the locus of the receptive field (superficial cutaneous, deep cutaneous or subcutaneous).2. SP-LI was determined using the avidin-biotin complex method and the relative staining intensity determined by image analysis. The possible significance of labelling intensity is discussed. Clear SP-LI appeared in twenty-nine of 117 dye-labelled neurones. All SP-LI positive units with identified receptive properties were nociceptive but not all categories of nociceptors were positive. The intensity of SP-LI labelling varied, often systematically, in relation to afferent properties. There was a tendency for nociceptive neurones with slower CVs and/or smaller cell bodies to show SP-LI.3. Nineteen of fifty-one C fibre neurones showed SP-LI. Fewer than half the C polymodal nociceptors (CPMs) were positive. The most intensely labelled units were the deep cutaneous nociceptors and some of the CPMs in glabrous skin. C low threshold mechanoreceptors and cooling-sensitive units did not show SP-LI.4. Ten of sixty six A fibre neurones exhibited SP-LI, including eight of sixteen AS nociceptors and two of fifteen A alpha/beta nociceptors. A fibre neurones exhibiting SP-LI included seven of eight deep cutaneous mechanical nociceptors and some superficial cutaneous mechano-heat nociceptors of hairy skin. In contrast, none of twenty superficial cutaneous A high threshold mechanoreceptor units or the thirty-five A fibre low threshold units (D-hair and other units) showed detectable SP-LI.5. We conclude that SP-LI labelling in guinea-pig DRG neurones is related to (a) afferent receptive properties, (b) the tissue in which the peripheral receptive terminals are located, (c) the CV and (d) the soma size.