Physiological and 15N-NMR analysis of molecular nitrogen fixation by Methanococcus thermolithotrophicus, Methanobacterium bryantii and Methanospirillum hungatei.
Physiological and 15N-NMR analysis of molecular nitrogen fixation by Methanococcus thermolithotrophicus, Methanobacterium bryantii and Methanospirillum hungatei.
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DOI：
10.1016/0167-4889(88)90138-3
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发表时间：
1988-10
期刊：
影响因子：
--
通讯作者：
N. Belay;R. Sparling;B. Choi;M. Roberts;J. Roberts;L. Daniels
中科院分区：
文献类型：
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作者：
N. Belay;R. Sparling;B. Choi;M. Roberts;J. Roberts;L. Daniels
用几种不同的实验方法证明了两种产甲烷中温细菌：甲烷杆菌MOH和嗜热甲烷螺杆菌GP1固定氮素。证据包括：(1)以N_2为唯一氮源的生长；(2)15N-核磁共振波谱检测到15N_2掺入细胞物质(包括可溶性氨基酸库和不溶性细胞蛋白和其他大分子)；(3)细胞将乙炔还原为乙烯，并用产甲烷抑制剂溴乙磺酸(BES)抑制这一反应。这些生物乙醇提取物的高分辨率15N-核磁共振分析和从这些提取物中提取的固体碎片的交叉极化魔角样品旋转分析与之前被确定为固定氮素的甲烷球菌的标记材料进行了比较。
Two mesophilic methanogenic bacteria,Methanobacterium bryantiistrain MOH andMethanospirillum hungateistrain GP1 were demonstrated, using several different experimental approaches, to fix dinitrogen. Evidence includes (1) growth with N2as the sole nitrogen source; (2) incorporation of15N2into cellular material (both soluble amino acid pools and insoluble cell protein and other macromolecules) detected by15N-NMR spectroscopy; (3) acetylene reduction to ethylene by the cells, and inhibition of this reaction by bromoethanesulfonic acid (BES), a methanogen inhibitor. High-resolution15N-NMR analysis of ethanol extracts of these organisms and cross-polarization magic-angle sample spinning analysis of the solid debris from these extracts are compared to labeled material fromMethanococcus thermolithotrophicus, a methanogen previously determined to fix dinitrogen.