Cell mediated immune response in sheep vaccinated with Brucella melitensis Rev. 1
Cell mediated immune response in sheep vaccinated with Brucella melitensis Rev. 1
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接种羊布鲁氏菌 Rev. 1 的绵羊的细胞介导的免疫反应
DOI：
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发表时间：
2007
影响因子：
0.4
通讯作者：
T. Goswami
中科院分区：
文献类型：
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作者：
D. Sinha;D. Singh;V. I. Bishor;T. Goswami
通过检测羊种布鲁氏菌Rev. 1疫苗免疫绵羊的淋巴细胞增殖反应和单核细胞来源的巨噬细胞产生的过氧化氢（H2 O2）和一氧化氮（NO），研究了羊种布鲁氏菌Rev. 1疫苗免疫绵羊的细胞介导免疫（CMI）应答。
三只绵羊接种了B。melitensis Rev.1（109 CFU），而将无菌PBS给予对照绵羊。在规定的时间间隔（接种后0 - 98天）分离外周血单核细胞，并在96个威尔斯孔组织培养板中用不同浓度的B培养48小时。羊种16 M可溶性抗原（BmSA）。采用MTT比色法检测淋巴细胞增殖活性。在接种后第14天至第84天，观察到接种组绵羊的刺激指数（SI）显著高于对照组绵羊。还估计了来自接种和对照绵羊的单核细胞衍生的巨噬细胞的H2 O2和NO产生。在整个研究期间，H2 O2和NO的产生都显着高于在对照组的免疫羊。该研究表明，接种疫苗刺激绵羊的显着CMI反应，如淋巴细胞的增殖和H2 O2的产生增加以及巨噬细胞的NO。
The cell mediated immune (CMI) response of the sheep vaccinated with Brucella melitensis Rev. 1 was studied by measuring the lymphocyte proliferation and estimation of hydrogen peroxide (H2O2) and nitric oxide (NO) from monocyte derived macrophages from control as well as Rev. 1 vaccinated sheep.
Three sheep were inoculated with B. melitensis Rev. 1 (109 CFU) while sterile PBS was given to control sheep. Peripheral blood monocytes were separated at defined interval (0 to 98 days post-vaccination) and cultured in 96 wells tissue culture plates for 48 h with different concentrations of B. melitensis 16 M soluble antigens (BmSA). The lymphoproliferation was estimated with 3-(4,5-dimethyl thiozol-2yl)-2,5 diphenyl tetrazolium bromide (MTT) colorimetric method. A significantly higher stimulation index (SI) in vaccinated as compared to control sheep from 14th to 84th day post-vaccination was observed. The H2O2 and NO productions by the monocyte-derived macrophages from the vaccinated and control sheep were also estimated. All through the study period, both H2O2 and NO production were significantly higher in vaccinated than in control sheep. The study revealed that vaccination stimulates significant CMI response in sheep as indicated by higher proliferation of the lymphocytes and increased production of H2O2 as well as NO by the macrophages.