CHARACTERIZATION AND NUCLEOTIDE-SEQUENCE OF A CHICKEN GENE ENCODING AN OPAL SUPPRESSOR TRANSFER-RNA AND ITS FLANKING DNA SEGMENTS
CHARACTERIZATION AND NUCLEOTIDE-SEQUENCE OF A CHICKEN GENE ENCODING AN OPAL SUPPRESSOR TRANSFER-RNA AND ITS FLANKING DNA SEGMENTS
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DOI：
10.1073/pnas.80.16.4940
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
EDEN, FC
中科院分区：
文献类型：
--
作者：
HATFIELD, DL;DUDOCK, BS;EDEN, FC
A naturally occurring opal suppressor serine t[transfer]RNA were purified from chicken liver and used as a probe to isolate the corresponding gene from a library of chicken DNA in bacteriophage .lambda.. This minor RNA is encoded by a single-copy gene that is not part of a tRNA gene cluster. DNA sequence analysis of the gene and its flanking DNA segments shows that the gene is encoded in an 87-base-pair segment without intervening sequences and specifies a tRNA that reads the termination codon UGA. This gene has additional nucleotides in the 5'' internal promoter region but has a normal 3'' internal promoter sequence and the usual termination signal.
