Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus
Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus
复制标题
生长分化因子 9 (Gdf9) 定位于先熟雌雄同体硬骨鱼（稻田鳗鲡）的雌性和雄性生殖细胞中
DOI：
10.1016/j.ygcen.2012.06.016
复制
发表时间：
2012-09-01
影响因子：
2.7
通讯作者：
Zhang, Weimin
中科院分区：
文献类型：
--
作者：
He, Zhi;Wu, Yangsheng;Zhang, Weimin
生长分化因子 9 (GDF9) 是转化生长因子 β (TGFb) 超家族的成员。作为卵母细胞衍生的生长因子，GDF9 在调节卵泡发育中发挥着关键作用。在本研究中，我们从黄鳝卵巢中鉴定出gdf9同源物，并在mRNA和蛋白质水平上分析了其表达。稻田鳗 Gdf9 与其他硬骨鱼，特别是鲈形目鱼类表现出高度同源性。 RT-PCR分析表明，黄鳝gdf9仅在卵巢和睾丸中表达。卵巢中gdf9的mRNA水平在卵黄发生前（PV）阶段显着增加，然后随着卵黄发生显着下降。在自然性别变化过程中，稻田鳗 gdf9 的表达在间性阶段达到顶峰。 Gdf9 的免疫反应性仅位于卵巢卵母细胞的细胞质中，特别是早期卵母细胞，但不在卵原细胞中。有趣的是，在间性性腺退化的卵母细胞中也检测到了强烈的免疫反应信号。此外，首次证明Gdf9免疫反应性位于硬骨鱼稻田鳗的精原细胞和精母细胞的细胞质中。综上所述，本研究结果表明 Gdf9 可能在稻田鳗的卵泡发生和精子发生中发挥重要作用。 (C) 2012 Elsevier Inc. 保留所有权利。
Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor beta (TGFb) superfamily. As an oocyte-derived growth factor, GDF9 plays key roles in regulating follicle development. In the present study, we identified a gdf9 homologue from the ovary of ricefield eel, and analyzed its expression both at the mRNA and protein levels. Ricefield eel Gdf9 showed high homologies with those of other teleosts, especially perciformes fish. RT-PCR analysis revealed that ricefield eel gdf9 was expressed exclusively in the ovary and testis. The mRNA levels of gdf9 in the ovary were increased significantly at the pre-vitellogenic (PV) stage and then decreased significantly along with vitellogenesis. During the natural sex change, expression of ricefield eel gdf9 was peaked at the intersexual stages. The immunoreactivity for Gdf9 was localized exclusively in the cytoplasm of the oocytes in the ovary, particularly the oocytes at early stages, but not in the oogonia. Interestingly, strong immunoreactive signals were also detected in the degenerating oocytes in the intersexual gonad. Furthermore, the Gdf9 immunoreactivity was demonstrated for the first time to be localized in the cytoplasm of spermatogonia and spermatocytes of ricefield eel, a teleost fish. Taken together, the results of present study suggested that Gdf9 may play important roles in the folliculogenesis as well as spermatogenesis in ricefield eels. (C) 2012 Elsevier Inc. All rights reserved.