Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system
Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system
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使用半完整哺乳动物细胞系统导入过氧化物酶体膜和基质蛋白
DOI：
10.1007/978-1-4939-6937-1_20
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Okumoto;K.;Honsho;M.;Liu;Y.;and Fujiki;Y.
过氧化物酶体是必需的细胞内细胞器，其催化许多必需的代谢途径，包括极长链脂肪酸的β-氧化、缩醛磷脂、胆汁酸的合成以及过氧化氢的产生和降解。这些过氧化物酶体的功能是通过催化这些反应的酶的严格和时空调节的区室化来实现的。过氧化物酶体蛋白输入缺陷导致人类遗传性过氧化物酶体生物合成障碍。过氧化物酶体基质和膜蛋白在游离核糖体上合成，并以依赖于其特异性靶向信号及其受体的方式转运至过氧化物酶体。过氧化物酶体蛋白输入可以使用半完整测定系统进行分析，其中靶向效率容易通过免疫荧光显微镜监测。此外，过氧化物酶体蛋白输入所需的胞质因子可以被操纵，这表明半完整系统是一个有用的和方便的系统，以揭示过氧化物酶体蛋白输入的分子机制。
Peroxisomes are essential intracellular organelles that catalyze a number of essential metabolic pathways including β-oxidation of very long chain fatty acids, synthesis of plasmalogen, bile acids, and generation and degradation of hydrogen peroxide. These peroxisomal functions are accomplished by strictly and spatiotemporally regulated compartmentalization of the enzymes catalyzing these reactions. Defects in peroxisomal protein import result in inherited peroxisome biogenesis disorders in humans. Peroxisomal matrix and membrane proteins are synthesized on free ribosomes and transported to peroxisomes in a manner dependent on their specific targeting signals and their receptors. Peroxisomal protein import can be analyzed using a semi-intact assay system, in which targeting efficiency is readily monitored by immunofluorescence microscopy. Furthermore, cytosolic factors required for peroxisomal protein import can be manipulated, suggesting that the semi-intact system is a useful and convenient system to uncover the molecular mechanisms of peroxisomal protein import.