Metabolism to methionine and growth stimulation by 5'-methylthioadenosine and 5'-methylthioinosine in mammalian cells.
Metabolism to methionine and growth stimulation by 5'-methylthioadenosine and 5'-methylthioinosine in mammalian cells.
复制标题
哺乳动物细胞中蛋氨酸的代谢和 5-甲硫腺苷和 5-甲硫肌苷的生长刺激。
DOI：
10.1016/0006-291x(83)91476-6
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发表时间：
1983
影响因子：
3.1
通讯作者：
Kamatani,N
中科院分区：
文献类型：
--
作者：
Carson,DA;Willis,EH;Kamatani,N
活的人和小鼠淋巴母细胞和正常的人体组织提取物将硫醚核苷5′-甲基硫代腺苷（MeSAdo）和5′-甲基硫代肌苷（MeSIno）转化为蛋氨酸。MeSAdo和MeSIno均支持人或小鼠淋巴母细胞在蛋氨酸缺乏培养基中的短期生长，但同型半胱氨酸不支持。然而，浓度大于25 μM的MeSAdo抑制细胞生长。MeSIno在浓度高达200 μM时无毒，并支持淋巴细胞在无蛋氨酸的培养基中长期生长。
Viable human and murine lymphoblasts, and normal human tissue extracts, converted the thioether nucleosides 5′-methylthioadenosine (MeSAdo) and 5′-methylthioinosine (MeSIno) to methionine. Both MeSAdo and MeSIno, but not homocysteine, supported the short-term growth of human or murine lymphoblasts in methionine deficient medium. However, MeSAdo at concentrations greater than 25 μM inhibited cell growth. MeSIno was non-toxic at concentrations up to 200 μM, and supported the long-term growth of lymphoblasts in methionine-free medium.