The Bacillus subtilis quorum-sensing molecule CSF contributes to intestinal Homeostasis via OCTN2, a host cell membrane transporter
The Bacillus subtilis quorum-sensing molecule CSF contributes to intestinal Homeostasis via OCTN2, a host cell membrane transporter
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DOI：
10.1016/j.chom.2007.05.004
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发表时间：
2007-06-01
影响因子：
30.3
通讯作者：
Chang, Eugene B.
中科院分区：
文献类型：
--
作者：
Fujiya, Mikihiro;Musch, Mark W.;Chang, Eugene B.
细菌利用群体感应分子（QSMs）在物种内部和物种之间进行交流。然而，QSMs对真核宿主细胞的作用却很少得到关注。我们报道革兰氏阳性杆菌枯草芽孢杆菌的群体感应五肽，能力和产孢因子（CSF）激活肠上皮细胞的关键生存途径，包括p38 MAP激酶和蛋白激酶B （Akt）。脑脊液还能诱导细胞保护性热休克蛋白（Hsps），防止氧化诱导的肠上皮细胞损伤和屏障功能丧失。脑脊液的这些作用取决于其被顶膜有机阳离子转运蛋白-2 （OCTN2）吸收。因此，octn2介导的脑脊液运输作为宿主-细菌相互作用的一个例子，允许宿主监测和响应结肠菌群行为或组成的变化。
Bacteria use quorum-sensing molecules (QSMs) to communicate within as well as across species. However, the effects of QSMs on eukaryotic host cells have received limited attention. We report that the quorum-sensing pentapeptide, competence and sporulation factor (CSF), of the Gram-positive bacterium Bacillus subtilis activates key survival pathways, including p38 MAP kinase and protein kinase B (Akt), in intestinal epithelial cells. CSF also induces cytoprotective heat shock proteins (Hsps), which prevent oxidant-induced intestinal epithelial cell injury and loss of barrier function. These effects of CSF depend on its uptake by an apical membrane organic cation transporter-2 (OCTN2). Thus, OCTN2-mediated CSF transport serves as an example of a host-bacterial interaction that allows the host to monitor and respond to changes in the behavior or composition of colonic flora.