Depletion of nicotinamide adenine dinucleotide in normal and xeroderma pigmentosum fibroblast cells by the antitumor drug CC-1065.
Depletion of nicotinamide adenine dinucleotide in normal and xeroderma pigmentosum fibroblast cells by the antitumor drug CC-1065.
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抗肿瘤药物 CC-1065 消耗正常和着色性干皮病成纤维细胞中的烟酰胺腺嘌呤二核苷酸。
DOI：
10.1021/bi00368a014
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Hurley,LH
中科院分区：
文献类型：
--
作者：
Jacobson,MK;Twehous,D;Hurley,LH
材料与方法材料。CC-1065是从D.Martin博士(密苏里州卡拉马祖Upjohn公司)获得的，在使用前溶解在DMF中，并如前所述进行测定(Swenson等人，1982)。细胞培养。正常人皮肤成纤维细胞(CCD-45SK，ATCC CRL 1506)和色素性干皮病(XP)细胞系XP12BE(ATCC CRL 1223互补组A)和XP6BE(ATCC CRL 1157互补组D)来自美国典型培养体系并按前面所述进行常规培养(Jacobson等，1983)。在实验中，细胞接种在35 mm的培养皿中，每周更换两次新鲜培养液进行培养，直到细胞达到融合。融合培养保存在含有如前所述的降低血清的培养基中。
Materials and Methods Materials. CC-1065 was obtained from Dr. D. Martin (Upjohn Company, Kalamazoo, MI) and was dissolved in DMF prior to use and assayed as before (Swenson et al., 1982). Cell Culture. Normal human skin fibroblasts (CCD-45SK, ATCC CRL 1506) and xeroderma pigmentosum (XP) cell lines XP12BE (ATCC CRL 1223 complementation group A) and XP6BE (ATCC CRL 1157 complementation group D) were obtained from the American Type Culture Collection and routinely cultured as described previously (Jacobson et al., 1983). For experiments, cells were seeded in 35-mm dishes and grown with replacement of fresh medium twice per week until they reached confluence. Confluent cultures were maintained in medium with reduced serum as described pre-