Loose Plant Architecture1, an INDETERMINATE DOMAIN Protein Involved in Shoot Gravitropism, Regulates Plant Architecture in Rice
Loose Plant Architecture1, an INDETERMINATE DOMAIN Protein Involved in Shoot Gravitropism, Regulates Plant Architecture in Rice
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松散植物结构1，一种参与芽向地性的不确定域蛋白，调节水稻的植物结构
DOI：
10.1104/pp.112.208496
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发表时间：
2013-01-01
期刊：
影响因子：
7.4
通讯作者：
Cheng, Zhukuan
中科院分区：
文献类型：
--
作者：
Wu, Xinru;Tang, Ding;Cheng, Zhukuan
分蘖角和叶片角是水稻植株结构的两个重要组成部分，对水稻产量起着至关重要的作用。本文报道了拟南芥（Arabidopsis thaliana）中AtIDD15/SHOOT GRAVITROPISM5 （SGR5）基因的功能同源基因——水稻松散植物结构1 （LPA1）基因的克隆和鉴定。LPA1通过控制分蘖节和叶节的近轴生长来调节分蘖角和叶片角。LPA1也影响茎部向地性。表达模式分析表明，LPA1通过影响叶鞘、叶柄和叶板关节的向地性来影响植物构型。然而，与LAZY1不同，LPA1仅通过调节淀粉体的沉降速率来影响胚芽向重力性的重力感知或信号转导。LPA1编码植物特异性INDETERMINATE DOMAIN蛋白，并定义了一个由28个INDETERMINATE DOMAIN蛋白组成的新亚家族，这些蛋白具有几个独特的保守特征。LPA1定位于细胞核，作为一种活跃的转录抑制因子，其活性主要由保守的乙烯反应因子相关的两亲性抑制样基序赋予。进一步分析表明，LPA1参与了一个复杂的转录和蛋白质相互作用网络，并进化出不同于SGR5的新功能。该研究不仅有助于对水稻向地性机制的理解，而且为水稻育种提供了有用的遗传材料。
Tiller angle and leaf angle are two important components of rice (Oryza sativa) plant architecture that play a crucial role in determining grain yield. Here, we report the cloning and characterization of the Loose Plant Architecture1 (LPA1) gene in rice, the functional ortholog of the AtIDD15/SHOOT GRAVITROPISM5 (SGR5) gene in Arabidopsis (Arabidopsis thaliana). LPA1 regulates tiller angle and leaf angle by controlling the adaxial growth of tiller node and lamina joint. LPA1 was also found to affect shoot gravitropism. Expression pattern analysis suggested that LPA1 influences plant architecture by affecting the gravitropism of leaf sheath pulvinus and lamina joint. However, LPA1 only influences gravity perception or signal transduction in coleoptile gravitropism by regulating the sedimentation rate of amyloplasts, distinct from the actions of LAZY1. LPA1 encodes a plant-specific INDETERMINATE DOMAIN protein and defines a novel subfamily of 28 INDETERMINATE DOMAIN proteins with several unique conserved features. LPA1 is localized in the nucleus and functions as an active transcriptional repressor, an activity mainly conferred by a conserved ethylene response factor-associated amphiphilic repression-like motif. Further analysis suggests that LPA1 participates in a complicated transcriptional and protein interaction network and has evolved novel functions distinct from SGR5. This study not only facilitates the understanding of gravitropism mechanisms but also generates a useful genetic material for rice breeding.