IMMUNOCYTOCHEMICAL LOCALIZATION OF GLUTAMATE-DECARBOXYLASE IN THE RAT BASOLATERAL AMYGDALOID NUCLEUS, WITH SPECIAL REFERENCE TO GABAERGIC INNERVATION OF AMYGDALOSTRIATAL PROJECTION NEURONS
IMMUNOCYTOCHEMICAL LOCALIZATION OF GLUTAMATE-DECARBOXYLASE IN THE RAT BASOLATERAL AMYGDALOID NUCLEUS, WITH SPECIAL REFERENCE TO GABAERGIC INNERVATION OF AMYGDALOSTRIATAL PROJECTION NEURONS
复制标题
DOI：
10.1002/cne.902730407
复制
发表时间：
1988-07-22
影响因子：
2.5
通讯作者：
CARLSEN, J
中科院分区：
文献类型：
--
作者：
CARLSEN, J
应用谷氨酸脱羧酶（GAD）免疫组织化学方法，在光镜和电镜水平对基底外侧杏仁核（BL）内的GABA能结构进行了定位。GAD免疫反应（GAD-IR）的染色模式包括点状结构和一组形态多样的GAD-IR神经元。在电子显微镜水平上，发现许多这些点状结构与细胞体以及未标记的远端部分（推测为投射和非投射神经元）形成对称的突触接触。此外，GAD免疫反应阳性神经元被确定在BL中，它们具有局部回路或内在神经元的超微结构特征，并且在腹侧纹状体注射后未被HRP逆行标记。这些GAD免疫活性神经元中的一些被GABA能终扣接触，形成对称的突触接触。通过GAD免疫组织化学结合高尔基体浸渍和辣根过氧化物酶（HRP）逆行追踪，在腹侧纹状体的嗅结节相关部位注射示踪剂，确定了BL中杏仁核投射神经元的GABA能神经支配。杏仁核纹状体的投射神经元在BL中观察到的连续性与梨状皮质的神经元，其投射到腹侧纹状体。结果提供了直接的证据，GAD-IR终扣的BL与确定的杏仁体纹状体投射神经元的突触接触的存在。本研究提供了直接的解剖学证据的生理观察，GABA表现出强大的调节杏仁核投射神经元在BL和借进一步支持的概念，皮质样功能组织的基底外侧杏仁核。
Glutamate decarboxylase (GAD) immunohistochemistry was employed at the light and electron microsocpic levels to localize GABAergic structures in the basolateral amygdaloid nucleus (BL). The GAD-immunoreactive (GAD-IR) staining pattern consisted of punctate structues and a morphologically diverse group of GAD-IR neurons. At the electron microscopic level many of these punctate structures were found to make symmetrical synaptic contacts with cell bodies as well as distal parts of unlabeled, presumably projection and nonprojection, neurons. In addition, GAD-immunoreactive neurons were identified in the BL, and they had the ultrastructural characteristics of local circuit or intrinsic neurons and were not retrogradely labeled with HRP following ventral striatal injections. Some of these GAD-immunoractive neurons were contacted by GABAergic boutons, forming symmetrical synaptic contacts. GABAergic innervation of amygdaloid projection neurons in the BL was identified by combining GAD immunohistochemistry with Golgi impregnation and retrograde tracing of horseradish peroxidase (HRP) following injections of the tracer in the olfactory-tubercle-related parts of the ventral striatum. Amygdalostriatal projection neurons in the BL were observed to be in continuity with neurons in the piriform cortex which project to the ventral striatum. The results provide direct evidence for the presence of GAD-IR boutons in the BL making synaptic contacts with identified amygdalostriatal projection neurons. The present study provides direct anatomical evidence for the physiological observation that GABA exhibits a powerful regulation of the amygdaloid projection neurons in the BL and lends further support to the concept of a corticallike functional organization of the basolateral amygdala.