Organization of projections from olfactory epithelium to olfactory bulb in the frog, Rana pipiens.
Organization of projections from olfactory epithelium to olfactory bulb in the frog, Rana pipiens.
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青蛙（Rana pipiens）从嗅觉上皮到嗅球的投射组织。
DOI：
10.1002/cne.902990304
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Gesteland,RC
中科院分区：
文献类型：
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作者：
Duncan,HJ;Nickell,WT;Shipley,MT;Gesteland,RC
嗅觉质量编码的一个假设是，嗅上皮的区域对特定的气味质量有差异的敏感性，并且这种区域敏感性通过嗅神经以地形学的方式传递到嗅球。这一假说的一个推论是，在上皮细胞和嗅球之间有一个足够有序的连接来传递这种地形编码。因此，我们研究了青蛙从上皮到嗅球的投射中的地形图，这是许多电生理学研究的主题，但尚未使用现代神经解剖学技术进行研究。示踪剂WGA-HRP应用于腹侧或背侧嗅上皮，或两者兼而有之。顺行运输的标签嗅球后，看到短短的2天，标签仍然存在于球注射后21天。在将WGA-HRP应用于整个上皮的情况下，同侧嗅球有密集的顺行标记。此外，一个小的对侧球的内侧部分被labeled.Injections限制到腹侧或背侧上皮产生的模式顺行标记在肾小球层的嗅球，这与注射的大小和位置不同。在背侧或腹侧上皮中进行非常大的注射时，标记似乎均匀分布在肾小球层中。较小的注射在腹侧上皮，有较重的标记在外侧比在肾小球层的内侧部分，虽然轻标记被发现在肾小球层的所有区域。相比之下，注射部位仅限于背侧上皮产生更多的顺行标记在内侧比外侧部分的肾小球层。这些模式延伸到整个球的背腹侧范围。在所使用的顺行追踪技术的限制范围内，我们无法检测到肾小球层中的标记模式与腹侧或背侧上皮中注射部位的内侧-外侧或吻侧-尾侧位置之间的任何系统关系。我们得出结论，在青蛙中，与其他两栖动物一样，在上皮和嗅球之间只有有限程度的拓扑有序性。
One hypothesis for the coding of olfactory quality is that regions of the olfactory epithelium are differentially sensitive to particular odor qualities and that this regional sensitivity is conveyed to the olfactory bulb in a topographic manner by the olfactory nerve. A corollary to this hypothesis is that there is a sufficiently orderly connection between the epithelium and the olfactory bulb to convey this topographical coding. Thus we examined topography in the projection from epithelium to bulb in the frog, which has been the subject of numerous electrophysiological studies but has not yet been examined using modern neuroanatomical techniques.The tracer WGA‐HRP was applied to the ventral or to the dorsal olfactory epithelium, or both. Anterograde transport of label to the olfactory bulb was seen after as few as 2 days; label was still present in the bulb as long as 21 days postinjection. In cases where WGA‐HRP was applied to the entire epithelium, there was dense anterograde labelling of the ipsilateral olfactory bulb. In addition, a small medial portion of the contralateral bulb was labelled.Injections limited to either the ventral or dorsal epithelium produced patterns of anterograde labelling in the glomerular layer of the olfactory bulb, which varied with the size and location of the injection. With very large injections in either the dorsal or ventral epithelium, label appeared to be evenly distributed in the glomerular layer. With smaller injections in the ventral epithelium, there was heavier labelling in the lateral than in the medial portions of the glomerular layer, although light labelling was found in all regions of the glomerular layer. In contrast, injection sites restricted to the dorsal epithelium produced more anterograde labelling in the medial than lateral portions of the glomerular layer. These patterns extended throughout the dorsal‐ventral extent of the bulb.Within the limits of the anterograde tracing technique used, we were unable to detect any systematic relationship between the pattern of labelling in the glomerular layer and the medial‐lateral or rostral‐caudal location of the injection site in either the ventral or dorsal epithelium.We conclude that in the frog, as in other amphibia, there is only a limited degree of topographic order between the epithelium and the olfactory bulb.