Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages
Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages
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DOI：
10.1016/s0165-5728(01)00315-0
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发表时间：
2001-07-02
影响因子：
3.3
通讯作者：
Markey, SP
中科院分区：
文献类型：
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作者：
Jiang, ZG;Piggee, C;Markey, SP
我们试图鉴定HIV-1感染的单核吞噬细胞分泌的神经毒素，这些毒素可能导致HIV-1相关痴呆（HAD）的病理生理学。神经毒性因子的特点是在一批条件培养基（CM）从人单核细胞衍生的巨噬细胞（MDM）感染HIV-1（ADA）和/或激活脂多糖（LPS）。所有神经毒性为：存在于< 3000-Da级分中;被5 M MK 801阻断;并且不是胰蛋白酶敏感的或可提取到极性有机溶剂中。谷氨酸测量CM占所有的神经毒性作用，观察到从HIV/LPS CM星形胶质细胞贫乏的神经元培养，并可能有助于HIV-1相关的痴呆症的病理生理。(C)2001 Elsevier Science B. V.保留所有权利。
We sought to identify neurotoxin(s) secreted by HIV-1-infected mononuclear phagocytes that could contribute to the pathophysiology of HIV-1-associated dementia (HAD). Neurotoxic factors were characterized in batches of conditioned media (CM) from human monocyte-derived macrophages (MDM) infected with HIV-1(ADA) and/or activated with lipopolysaccharide (LPS). All of the neurotoxicity was: present in the < 3000-Da fraction; blocked by 5 M MK801; and not trypsin sensitive or extractable into polar organic solvents. Glutamate measured in CM accounted for all neurotoxic effects observed from HIV/LPS CM in astrocyte-poor neuronal cultures and may contribute to the pathophysiology of HIV-1-associated dementia. (C) 2001 Elsevier Science B.V. All rights reserved.