Sperm quality, egg size, fecundity and their relationships with fertilization rate of grass carp (Ctenopharyngodon idella)
Sperm quality, egg size, fecundity and their relationships with fertilization rate of grass carp (Ctenopharyngodon idella)
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草鱼精子质量、卵子大小、繁殖力及其与受精率的关系
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
F. Öğretmen
中科院分区：
文献类型：
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作者：
Yusuf Bozkurt;F. Öğretmen
用腹部按摩的方法采集了15尾麻醉的雌雄草鱼的配子。采集的精液中，精子体积为15.64±5.39mL，精子活动率为81.20±7.61%，活动时间为62.06±19.40s，密度为17.88±5.48×109，总密度为428.90±302.05×109，pH为7.25±0.81。雌性草鱼的卵大小(Mm)和繁殖力(卵/鱼)分别为417867±0.028和1.04±36.274。在相同的卵池中检测精子的受精能力。每次受精的受精量为2×10~5spz/枚。受精率为79.3±2.95%，与精子活动率(r=0.932)、卵子大小(r=0.513)和受精率(r=0.539)呈正相关。
Gametes were collected by abdominal massage from 15 anesthesized male and female grass carp. In collected sperm samples the following mean spermatological properties were determined: sperm volume 15.64 ± 5.39 mL, sperm motility 81.20 ± 7.61%, motility duration 62.06 ± 19.40s, density 17.88±5.48 x10 9 mL -1 , total density 428.90±302.05 x10 9 , and pH 7.25±0.81. Also egg size (mm) and fecundity (egg/fish) were determined as 1.04±0.028 and 417867 ± 36.274 respectively in female grass carp. The fertilization capacity of sperm was tested with the same egg pool. The insemination dosage was 2x10 5 spz/egg for each fertilization experiment. Fertilization rate was determined as mean 79.3±2.95% and positively correlated with sperm motility (r=0.932, p<0.01), egg size (r=0.513, p>0.05) and fecundity (r=0.539, p>0.05).