Soil CO2 respiration:: Comparison of chemical titration, CO2 IRGA analysis and the Solvita gel system
Soil CO2 respiration:: Comparison of chemical titration, CO2 IRGA analysis and the Solvita gel system
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DOI：
10.1017/s174217050800224x
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发表时间：
2008-06-01
影响因子：
2.7
通讯作者：
Evans, E.
中科院分区：
文献类型：
--
作者：
Haney, R. L.;Brinton, W. F.;Evans, E.
土壤二氧化碳呼吸的测量是衡量土壤生物肥力的一种手段。实验室中采用的呼吸测试方法有所不同，迄今为止所需的设备和劳动力在一定程度上限制了此类方法的更广泛采用。本研究的目的是比较三种方法测量土壤CO(2)呼吸的结果：（1）滴定法； (2)红外气体分析(IRGA)； (3) 用于土壤 CO(2) 分析的 Solvita 凝胶系统。我们从美国各地采集了 36 个土壤样本进行比较，其 pH 值范围为 4.5 至 8.5，有机碳含量为 0.8% 至 4.6%，粘土含量为 6% 至 62%。孵育 24 小时后，所有三种方法均高度相关（滴定和 Solvita r(2) = 0.82，呼吸计和 Solvita r(2) = 0.79，滴定与呼吸计 r(2) = 0.95）。所有三种方法的 24 小时（1 天）CO(2) 释放量也与基础土壤呼吸（7-28 天）和累积 28 天 CO(2) 呼吸高度相关。在原来的 36 个土壤样本的基础上，又采集了 24 个土壤样本，总共 60 个土壤样本。这些样品用于使用 CO(2) 滴定结果和回归分析来校准 Solvita 凝胶数字读色器结果。回归分析得出方程 y = 20.6 * (Solvita 数) - 16.5，r(2) 为 0.83。数据表明，用于土壤 CO(2) 分析的 Solvita 凝胶系统可能是一种简单且易于使用的量化土壤微生物活性的方法。凝胶系统也可用于表面气室中的原位测量。一旦建立了标准化的土壤采样和实验室分析方案，Solvita 方法就可以轻松地应用于商业土壤测试实验室，作为土壤微生物活动的指标。
The measurement of soil carbon dioxide respiration is a means to gauge biological soil fertility. Test methods for respiration employed in the laboratory vary somewhat, and to date the equipment and labor required have somewhat limited more widespread adoption of such methodologies. The purpose of this research is to compare the results of measured soil CO(2) respiration using three methods: (1) titration method; (2) infrared gas analysis (IRGA); and (3) the Solvita gel system for soil CO(2) analysis. We acquired 36 soil samples from across the USA for comparison, which ranged in pH from 4.5 to 8.5, organic C from 0.8 to 4.6% and the clay content from 6 to 62%. All three methods were highly correlated with each other after 24-h of incubation (titration and Solvita r(2) = 0.82, respirometer and Solvita r(2) = 0.79 and titration versus respirometer r(2) = 0.95). The 24-h (1-day) CO(2) release from all three methods was also highly correlated to both basal soil respiration (7-28 days) and cumulative 28-day CO(2) respiration. An additional 24 soil samples were acquired and added to the original 36, for a total of 60 soil samples. These samples were used for calibration of the Solvita gel digital color reader results using CO(2)-titration results and regression analysis. Regression analysis resulted in the equation y = 20.6 * (Solvita number) - 16.5 with an r(2) of 0.83. The data suggest that the Solvita gel system for soil CO(2) analysis could be a simple and easily used method to quantify soil microbial activity. Applications may also exist for the gel system for in situ measurements in surface gas chambers. Once standardized soil sampling and laboratory analysis protocols are established, the Solvita method could be easily adapted to commercial soil testing labs as an index of soil microbial activity.