Study on the redox reactions for organic dyes and S-nitrosylated peptide in electrospray droplet impact
Study on the redox reactions for organic dyes and S-nitrosylated peptide in electrospray droplet impact
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DOI：
10.1002/jms.1521
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发表时间：
2009-04-01
影响因子：
2.3
通讯作者：
Hiraoka, Kenzo
中科院分区：
文献类型：
--
作者：
Asakawa, Daiki;Hiraoka, Kenzo
电离过程中分析物的还原常常使分子结构的测定变得模糊不清。分析物的还原可以通过各种解吸/电离方法进行，如快原子轰击（FAB）、二次离子质谱（西姆斯）、基质辅助激光解吸/电离（MALDI）和多孔硅解吸电离（DIOS）。还原敏感染料和S-亚硝基化肽通过EDI分析。未观察到亚甲蓝减少。虽然甲基红具有比亚甲基蓝低的还原电位，但检测到还原产物离子。对于S-亚硝基化的蛋白质，EDI中可观察到质子化的分子离子[M + H]（+）和NO消除的分子离子[M-NO+H]（+），但与MALDI相比，EDI中的还原反应受到很大抑制。因此，所检测的分析物在EDI中几乎不发生还原反应。版权所有（C）2008约翰威利父子有限公司
Reduction of analytes in ionization processes often obscures the determination of molecular structure. The reduction of analytes is found to take place in various desorption/ionization methods such as fast atom bombardment (FAB), secondary ion mass spectrometry (SIMS), matrix-assisted laser desorption/ionization (MALDI) and desorption ionization on porous silicon (DIOS).To examine the extent of the reduction reactions taking place in electrospray droplet impact (EDI) processes, reduction-sensitive dyes and S-nitrosylated peptide were analyzed by EDI. No reduction was observed for methylene blue. While methyl red has a lower reduction potential than methylene blue, the reduction product ions were detected. For S-nitrosylated pepticle, protonated molecule ion (M + H](+) and NO-eliminated molecular ion [M-NO+H](+) were observed but reduction reactions are largely suppressed in EDI compared with that in MALDI. As such, the analytes examined suffer from little reduction reactions in EDI. Copyright (C) 2008 John Wiley & Sons, Ltd.