Association of IL8 -105G/A with Mastitis Somatic Cell Score in Chinese Holstein Dairy Cows
Association of IL8 -105G/A with Mastitis Somatic Cell Score in Chinese Holstein Dairy Cows
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IL8 -105G/A与中国荷斯坦奶牛乳腺炎体细胞评分的关系
DOI：
10.1080/10495398.2014.939657
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发表时间：
2015-04-03
影响因子：
3.7
通讯作者：
Mao, Yongjiang
中科院分区：
文献类型：
--
作者：
Chen, Renjin;Wang, Zhenzhen;Mao, Yongjiang
采用聚合酶链式反应-单链构象多态性(PCR-SSCP)技术，对上海某奶牛场35个公牛家系的810头中国荷斯坦奶牛IL-8基因5‘上游的单核苷酸多态性(SNPs)进行了研究。采用实时荧光定量聚合酶链式反应(Real-time PCR)和蛋白质印迹法(Western印迹)检测不同基因型中国荷斯坦奶牛的mRNA和蛋白质水平。结果检测到1个SNP-105G>；A，分别为GG、GA和AA，其频率分别为0.38、0.46和0.16。SNP-105G>；A与体细胞评分(SCS)显著相关。GG型SCS显著低于GA、AA(P<0.05)。
The single nucleotide polymorphisms (SNPs) in the 5 ' upstream of bovine IL8 gene were investigated in 810 Chinese Holstein cows from 35 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. The Real-time PCR and Western blot were used to detect the mRNA and protein levels of genotype Chinese Holstein dairy cows. The results showed that one SNP -105G>A was detected, designating three genotypes (GG, GA and AA) with respective frequencies of 0.38, 0.46, and 0.16. The significant association of the SNP -105G>A with somatic cell score (SCS) was identified. Genotype GG had a significantly lower SCS than genotype GA or AA (P