Comparative analysis of early H2O2 accumulation in compatible and incompatible wheat-powdery mildew interactions
Comparative analysis of early H2O2 accumulation in compatible and incompatible wheat-powdery mildew interactions
复制标题
DOI：
10.1111/j.1365-3059.2005.01173.x
复制
发表时间：
2005-06-01
期刊：
影响因子：
2.7
通讯作者：
Jia, JZ
中科院分区：
文献类型：
--
作者：
Li, AL;Wang, ML;Jia, JZ
比较分析了过氧化氢（H2O2）积累对白粉病（Blumeria graminis[Erysiphe graminis] f.sp）的响应。小麦（Triticum aestivum）初生胚管（PGT）与附着胚管（AGT）接触，以及乳突（细胞壁附着）和超敏反应（HR）。利用3个小麦敏感品系（百农3217、北京837、京双16）和5个抗性品系[马德勒（Pm2 + Pm6）]的初生叶；Ulka/8 × Cc (Pm2)；马德勒/7 ×百农3217 (Pm2)；Pm16;Pm16/7 × Beijing 837 (Pm16)]，接种后10 ~ 48 h早期H2O2积累情况进行了研究。在小麦敏感品系和抗性品系的有效乳突和相关细胞质囊泡中均发现了较强的H2O2积累，这表明H2O2在有效乳突形成过程中发挥了重要作用，是植物抵御白粉病的一般防御手段。在所有5个抗性品系中均观察到有效乳头形成的高频率。在耐药品系中，马德勒和乌尔卡/8 × Cc比Pm16和Pm16/7 ×北京837更早发现超敏细胞死亡。在所有病例中，这都与H2O2在受攻击的表皮细胞中的积累有关。有趣的是，在Mardler/7 x百农3217系中，Pm2基因似乎介导了穿透抗性，而不是HR，这表明在这种遗传背景下，Pm2可能控制着HR独立的防御途径。由于有效的乳头和HR并不发生在同一细胞中，因此乳头沉积可能独立于HR反应，尽管这两种防御机制都与高H2O2积累有关。在小麦白粉病不相容的相互作用中，当乳突防御失败时，HR作为第二道防线来控制感染。
Comparative analysis was carried out for hydrogen peroxide (H2O2) accumulation in response to powdery mildew (Blumeria graminis[Erysiphe graminis] f.sp. tritici) primary germ tube (PGT) and appressorial germ tube (AGT) contact, and in papilla (cell wall apposition) and hypersensitive responses (HR) in wheat (Triticum aestivum). Using primary leaves of three susceptible wheat lines (Bainong 3217; Beijing 837; Jingshuang 16) and five resistant lines [Mardler (Pm2 + Pm6); Ulka/8 x Cc (Pm2); Mardler/7 x Bainong 3217 (Pm2); Pm16; Pm16/7 x Beijing 837 (Pm16)], early H2O2 accumulation between 10 and 48 h after inoculation was studied. Strong H2O2 accumulation was found in effective papillae and associated cytosolic vesicles in both susceptible and resistant wheat lines, suggesting the important role of H2O2 in effective papillae formed as a general plant defence against powdery mildew. High frequency of effective papilla formation was observed in all five resistant lines. Among resistant lines, hypersensitive cell death was detected earlier in Mardler and Ulka/8 x Cc than in Pm16 and Pm16/7 x Beijing 837. In all cases this was associated with H2O2 accumulation in attacked epidermal cells. Interestingly, penetration resistance but not HR appeared to be mediated by the Pm2 gene in the Mardler/7 x Bainong 3217 line, suggesting that Pm2 may govern an HR-independent defence pathway in this genetic background. As effective papillae and HR did not occur in the same cells, papilla deposition may be independent of the HR response, despite the fact that both defence mechanisms were associated with high H2O2 accumulation. In wheat powdery mildew-incompatible interactions, HR acts as a second line of defence to contain infection when the papilla defence fails.