Tianma modulates blood vessel tonicity.
Tianma modulates blood vessel tonicity.
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DOI：
10.2174/1874091x01206010056
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Heese K
中科院分区：
文献类型：
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作者：
Feng L;Manavalan A;Mishra M;Sze SK;Hu JM;Heese K
天马是一种传统的中药（TCM），通常用于治疗高血压和心脏病。为了阐明天田在分子水平上的功能，我们研究了天马对血管功能和主动脉蛋白代谢的影响。我们发现，一岁大鼠在长期治疗（〜2.5g/kg/day三个月）可以增强乙酰胆碱（ACH）诱导的内皮 - 独立性主动脉环（80 mm）的内皮胸间主动脉环（80 mm） ） - 和苯肾上腺素（PE）诱导的收缩。通过使用ITRAQ（相对和绝对定量）技术的ITRAQ（等速标签），我们从蛋白质组水平的功能数据中证实，天马治疗下调了收缩蛋白（例如ACTA2）和其他相关结构蛋白（例如Desmin）的表达并上调细胞外基质（ECM）糖蛋白（例如FBLN5）和主动脉组织中的抗血栓形成蛋白（例如Anxa2）。通过诱导推理，天马不仅可以通过抑制血管平滑肌收缩，而且通过增强血管弹性并稳定动脉结构来执行其血管舒张作用。因此，天马可能通过调节主动脉蛋白质组代谢而成为一种新型的心血管疾病治疗性草药。
Tianma is a traditional Chinese medicine (TCM) often used for the treatment of hypertension and heart diseases. To elucidate the function of tianma at the molecular level, we investigated the effect of tianma on vascular functions and aortic protein metabolism. We found that long-term treatment with tianma (~2.5g/kg/day for three months) in one-year-old rats could enhance acetylcholine (ACh)-induced vasorelaxation in endothelium-intact thoracic aortic rings against both KCl (80 mM)- and phenylephrine (PE)-induced contraction. By using the iTRAQ (isobaric tag for relative and absolute quantification) technique, we confirmed from the functional data at the proteome level that tianma treatment down-regulated the expressions of contractile proteins (e.g. Acta2) and other related structural proteins (e.g. desmin), and up-regulated the expressions of extracellular matrix (ECM) glycoproteins (e.g. Fbln5) and anti-thrombotic proteins (e.g. Anxa2) in aortic tissue. By inductive reasoning, tianma could perform its vasodilatory effect not only by inhibiting vascular smooth muscle contraction, but also by enhancing blood vessel elasticity and stabilizing the arterial structure. Thus, tianma might become a novel therapeutic herbal medicine for cardiovascular diseases by regulating the aortic proteome metabolism.