RNA polymerase of influenza virus. IV. Catalytic properties of the capped RNA endonuclease associated with the RNA polymerase.
RNA polymerase of influenza virus. IV. Catalytic properties of the capped RNA endonuclease associated with the RNA polymerase.
复制标题
流感病毒的RNA聚合酶。
DOI：
10.1093/nar/11.11.3637
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发表时间：
1983
影响因子：
14.9
通讯作者：
A. Ishihama
中科院分区：
文献类型：
--
作者：
K. Kawakami;K. Mizumoto;A. Ishihama
使用5'末端含有32 P标记CAP结构的合成杂聚物和均聚物分析了与流感病毒RNA聚合酶相关的加帽RNA特异性内切核酸酶的催化性质。如果底物RNA含有CAP-1结构（m7 GpppGm）和距离CAP结构9至11个核苷酸的A或U残基，则核酸内切酶显示其固有活性。二核苷酸ApG、游离CAP结构和不含CAP结构的RNA在不同程度上抑制了核酸内切酶的活性，这进一步支持了核酸内切酶对多种RNA信号的独立识别。在存在四种核糖核苷5 '-三磷酸的情况下，具有CAP-1结构的内切核酸裂解片段被掺入多核苷酸中，支持它们用作转录引物的概念。与引物连接的初始核苷酸是G残基，即与vRNA区段的3'末端的第二个碱基互补的核苷酸。
Catalytic properties of the capped RNA-specific endonuclease associated with the influenza virus RNA polymerase were analyzed with use of synthetic hetero- and homopolymers containing 32P-labeled CAP structures at their 5' termini. The endonuclease displays its intrinsic activity provided that substrate RNA contains both the CAP-1 structure (m7GpppGm) and either A or U residues at 9 to 11 nucleotides distant from the CAP structure. Independent recognition of multiple RNA signals by the endonuclease was further supported by the findings that dinucleotide ApG, free CAP structures and RNA without the CAP structure inhibited the endonuclease activity to different extents. In the presence of four species of ribonucleoside 5'-triphosphates, the endonucleolytically cleaved fragments with the CAP-1 structure were incorporated into polynucleotides, supporting the concept that they are used as the primers for the transcription. The initial nucleotide linked to the primers was a G residue, the nucleotide complementary to the second base of the 3' termini of the vRNA segments.