Strong static magnetic field effects on yeast proliferation and distribution
Strong static magnetic field effects on yeast proliferation and distribution
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DOI：
10.1016/j.bioelechem.2004.04.002
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发表时间：
2004-12-01
影响因子：
5
通讯作者：
Ueno, S
中科院分区：
文献类型：
--
作者：
Iwasaka, M;Ikehata, M;Ueno, S
本文主要研究了梯度磁场对酵母细胞增殖和质量分布等行为的影响，并评价了磁场对酵母培养系统中材料的影响。在磁场（通量密度B = 14 T）下在液体培养基中孵育酵母，酿酒酵母。将酵母菌置于磁场强度为9-14 T，磁场最大密度梯度为dB/dx = 94 T/m（x为空间坐标）的磁场中，培养16 T后，酵母菌的增殖率比对照组有所下降。研究了酵母培养系统的物理性质，以发现磁场下酵母增殖减速的机制。在暴露于磁场和不暴露于磁场的情况下比较酵母培养瓶中的气压。结果表明，与对照管内的压力相比，具有6 T、60 Pin的烧瓶内的气体压力缓慢增加。由于水（培养基溶液）和酵母的抗磁性，在梯度磁场作用下，液面明显倾斜，抗磁性力增强了悬浮液中的静水压力。此外，磁泳的酵母细胞在培养基中的解决方案表现出本地化的酵母沉降模式。磁改变气体运输过程中的作用，静水压力作用于酵母，和酵母沉淀的分布中的变化，以及磁场对酵母呼吸系统中观察到的干扰增殖的可能影响进行了讨论。(C)2004 Elsevier B. V.保留所有权利。
The present study focuses on the effects of gradient magnetic fields on the behavior of yeast, such as its proliferation and mass distribution, and evaluates the effects of magnetism on materials in the yeast culture system. Yeast, Saccharomyces cerevisiae, was incubated in a liquid medium under magnetic fields (flux density B = 14 T). When yeast in a tube was exposed to 9-14 T magnetic fields with a maximum flux density gradient of dB/dx = 94 T/m, where x is the space coordinate, the rate of yeast proliferation under the magnetic fields decreased after 16 It of incubation compared to that of the control group. The physical properties of the yeast culture system were investigated to discover the mechanism responsible for the observed deceleration in yeast proliferation under magnetic fields. Gas pressure inside the yeast culture flask was compared with and without exposure to a magnetic field. The results suggested that the gas pressure inside a flask with 6 T, 60 Pin slowly increased in comparison to the pressure inside a control tube. Due to the diamagnetism of water (medium solution) and yeast, the liquid surface distinctly inclined under gradient magnetic fields, and the hydrostatic force in suspension was strengthened by the diamagnetic forces. In addition, magnetophoresis of the yeast cells in the medium solution exhibited localization of the yeast sedimentation pattern. The roles of magnetically changed gas-transport processes, hydrostatic pressures acting on the yeast, and changes in the distribution of the yeast sedimentation, as well as the possible effects of magnetic fields on yeast respiratory systems in the observed disturbance of the proliferation are discussed. (C) 2004 Elsevier B.V. All rights reserved.