Analyses of chicken sialyltransferases related to O-glycosylation
Analyses of chicken sialyltransferases related to O-glycosylation
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DOI：
10.1016/j.jbiosc.2016.03.017
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发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Iijima, Shinji
中科院分区：
文献类型：
--
作者：
Kidani, Shunsuke;Kaneoka, Hidenori;Iijima, Shinji
克隆了鸡β -半乳糖苷α 2,3-唾液基转移酶1、2和5 （ST3Gal1、2和5）基因，并在293FT细胞中表达。ST3Gal1和2对半乳糖- 1,3- n -乙酰半乳糖胺和半乳糖- 1,3- n -乙酰氨基葡萄糖胺表现出酶活性。ST3Gal5仅对乳糖神经酰胺表现出活性。ST3Gal1和2以及先前克隆的ST3Gal3和6将cmp -唾液酸转移到asialofetuin中。逆转录定量PCR结果显示，ST3Gal1在气管、肺、脾和大鼠中表达量较高，而ST3Gal5在脾、大鼠和小肠中表达量较强。分泌蛋清蛋白的大鼠小管腺细胞中也有ST3Gal1、5和6的表达。ST3Gal1、5和6在卵细胞绒毛尿囊膜中表达，流感病毒在卵细胞绒毛尿囊膜中繁殖以生产疫苗。(C) 2016，生物技术学会，日本。版权所有。
The chicken beta-galactoside alpha 2,3-sialyltransferase 1, 2, and 5 (ST3Gal1, 2, and 5) genes were cloned, and their enzymes were expressed in 293FT cells. ST3Gal1 and 2 exhibited enzymatic activities toward galactose-beta 1,3-N-acetylgalactosamine and galactose-beta 1,3-N-acetylglucosamine. ST3Gal5 only exhibited activity toward lactosylceramide. ST3Gal1 and 2 and previously cloned ST3Gal3 and 6 transferred CMP-sialic acid to asialofetuin. Reverse-transcription-quantitative PCR indicated that ST3Gal1 was expressed at higher levels in the trachea, lung, spleen, and magnum, and the strong expression of ST3Gal5 was observed in the spleen, magnum, and small and large intestines. ST3Gal1, 5, and 6 were also expressed in the tubular gland cells of the magnum, which secretes egg-white proteins. ST3Gal1, 5, and 6 were expressed in the egg chorioallantoic membrane, in which influenza viruses are propagated for the production of vaccines. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.