A rapid screening procedure to identify mini-Tn10 insertion mutants of Escherichia coli K-12 with altered adhesion properties
A rapid screening procedure to identify mini-Tn10 insertion mutants of Escherichia coli K-12 with altered adhesion properties
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DOI：
10.1016/0378-1097(96)00237-6
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发表时间：
1996-08-15
影响因子：
2.1
通讯作者：
Bauda, P
中科院分区：
文献类型：
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作者：
Genevaux, P;Muller, S;Bauda, P
以聚苯乙烯96孔板为粘附表面，建立了一种快速筛选大肠杆菌粘附能力改变突变株的方法。在该测定期间，细菌菌株同时生长和粘附，并且在结晶紫染色后测量粘附的细胞。从总共7000个E. coliK-12 W3110中，筛选出50株粘附率低于40%的粘附缺陷型突变体，22株粘附率在40- 75%之间。对这72个突变体进行运动性测定，34个显示运动性改变。
A rapid screening procedure was developed for detection of Escherichia coli mutants with altered adhesion abilities using polystyrene 96-well microtiter plates as attachment surfaces. During this assay, bacterial strains grew and adhered simultaneously, and attached cells were measured after crystal violet staining. Starting with a total of 7000 W3110::Tn10 insertion mutants of E. coli K-12 W3110, 50 adhesion-deficient mutants were isolated which showed less than 40% attachment, and 22 mutants were found with an attachment of 40-75%. Motility assays were performed on these 72 mutants, and 34 displayed altered motility.