Expression of CCR9 β-chemokine receptor is modulated in thymocyte differentiation and is selectively maintained in CD8+ T cells from secondary lymphoid organs
Expression of CCR9 β-chemokine receptor is modulated in thymocyte differentiation and is selectively maintained in CD8+ T cells from secondary lymphoid organs
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DOI：
10.1182/blood.v97.4.850
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发表时间：
2001-02-15
期刊：
影响因子：
20.3
通讯作者：
Márquez, G
中科院分区：
文献类型：
--
作者：
Carramolino, L;Zaballos, A;Márquez, G
趋化因子似乎在淋巴祖细胞在胸腺内的播种、成熟T细胞在该器官内的协调运动的调节以及由此产生的幼稚T细胞向次级淋巴器官的出口中起着重要作用。CCR9是趋化因子TECK/CCL25的特异性受体，在胸腺、淋巴结和脾脏中选择性表达。使用特异性抗CCR9多克隆抗体K629和半定量逆转录聚合酶链反应程序，详细研究了CCR9在胸腺和次级淋巴器官中的表达。结果表明，CD4(+)CD8（+）双阳性胸腺细胞在胸腺中CCR9表达量最高。单阳性CD8（+）胸腺细胞在作为成熟幼稚T细胞放弃胸腺后继续表达该受体，这表明存在CD8(+)CD69（低）CD62L（高）CCR9（+）细胞亚群。与此一致的是，来自淋巴结、脾脏和Peyer斑块的CD8（+）淋巴细胞表达功能性CCR9，因为其表达与CCL25的迁移相关。相反，CD4（+）胸腺细胞在放弃胸腺之前会丢失CCR9，来自次级淋巴器官的CD4(+) T细胞也缺乏CCR9的表达。对不同年龄小鼠胸腺细胞中CCR9表达的分析表明，CCR9水平受年龄的影响，因为该受体更丰富，其对CCL25的反应在新生动物中更强。总的来说，这些结果表明CCR9在小鼠一生中胸腺细胞发育中起作用，在CD4（+）和CD8（+）谱系之间存在明显差异。(C) 2001年由美国血液学会出版。
Chemokines appear to have an important role in the seeding of lymphoid progenitors in the thymus, the regulation of the coordinated movements of the maturing T cells within this organ, end the egress of the resulting naive T cells to secondary lymphoid organs. CCR9, the specific receptor for the beta -chemokine TECK/CCL25, is selectively expressed in thymus, lymph node, and spleen. Using a specific anti-CCR9 polyclonal antibody, K629, and a semiquantitative reverse transcriptase-polymerase chain reaction procedure, a detailed study of CCR9 expression in the thymus and secondary lymphoid organs was performed. The results show that CD4(+)CD8(+) double-positive thymocytes have the highest CCR9 expression in thymus. Single-positive CD8(+) thymocytes continue to express this receptor after abandoning the thymus as mature naive T cells, as suggested by the existence of a CD8(+)CD69(low)CD62L(high)CCR9(+) cell subset. Consistent with this, CD8(+) lymphocytes from lymph nodes, spleen, and Peyer patches express a functional CCR9, as its expression correlates with migration in response to CCL25. Conversely, CD4(+) thymocytes lose CCR9 before abandoning the thymus, and CD4(+) T cells from secondary lymphoid organs also lack CCR9 expression. Analysis of CCR9 expression in thymocytes from mice of different ages showed that CCR9 levels are affected by age, as this receptor is more abundant, and its response to CCL25 is more potent in newborn animals. Collectively, these results suggest that CCR9 has a role in thymocyte development throughout murine life, with clear differences between the CD4(+) and CD8(+) lineages. (C) 2001 by The American Society of Hematology.