Colored Potato Extracts Induce Superoxide Dismutase-2 mRNA Via ERK1/2 Pathway in HepG2 Cells
Colored Potato Extracts Induce Superoxide Dismutase-2 mRNA Via ERK1/2 Pathway in HepG2 Cells
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DOI：
10.1007/s11130-010-0171-7
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发表时间：
2010-09-01
影响因子：
4
通讯作者：
Fukushima, Michihiro
中科院分区：
文献类型：
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作者：
Hashimoto, Naoto;Noda, Takahiro;Fukushima, Michihiro
喂食含有花青素丰富的马铃薯品种影子女王(SQ)的老鼠，先前显示出肝脏超氧化物歧化酶(SOD)-2mRNA水平的增加。我们研究了SQ提取物是否会直接增加HepG2细胞中肝组织SOD-2的mRNA水平。此外，我们还估计了诱导SOD-2mRNA表达的细胞内信号通路。SQ等四种作物提取物刺激HepG2细胞12h，只有彩色马铃薯提取物能显著诱导细胞表达SOD-2mRNA。细胞外信号相关激酶(ERK)1/2通路的阻断剂可阻断这种对SOD-2mRNA表达的诱导。此外，SQ提取物在刺激15或30分钟后可增加ERK1/2的磷酸化。这些结果表明，SQ提取物通过激活ERK1/2途径直接诱导HepG2细胞中肝组织SOD-2mRNA的表达。
Rats fed a diet containing Shadow Queen (SQ), an anthocyanin-rich potato cultivar, previously showed an increase in the hepatic superoxide dismutase (SOD)-2 mRNA level. We investigated whether an extract of SQ would directly increase the hepatic SOD-2 mRNA level in HepG2 cells. Furthermore, we estimated the intracellular signaling pathway for the induction of SOD-2 mRNA expression. HepG2 cells were stimulated using extracts of four crops, including SQ, for 12 h; only extracts of colored potatoes induced SOD-2 mRNA expression significantly. This induction of SOD-2 mRNA expression was blocked by an inhibitor of the extracellular signal-related kinase (ERK) 1/2 pathway. Furthermore, an extract of SQ increased the phosphorylation of ERK1/2 after 15 or 30 min of stimulation. These data indicate that an extract of SQ directly induces hepatic SOD-2 mRNA expression via activation of ERK1/2 pathway in HepG2 cells.