The response of cord blood megakaryocyte progenitors to IL‐3, IL‐6 and aplastic canine serum varies with gestational age
The response of cord blood megakaryocyte progenitors to IL‐3, IL‐6 and aplastic canine serum varies with gestational age
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脐带血巨核细胞祖细胞对IL-3、IL-6和再生障碍犬血清的反应随胎龄而变化
DOI：
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发表时间：
1995
影响因子：
6.5
通讯作者：
A. Eldor
中科院分区：
文献类型：
--
作者：
V. Deutsch;Tom Olson;A. Nagler;S. Slavin;R. Levine;A. Eldor
胎儿脐带血（CB）富含造血干细胞和祖细胞。我们研究了不同胎龄CB中巨核细胞（MK）祖细胞对各种细胞因子：IL-3、IL-6、IL-3 + IL-6和再生障碍性犬血清（PICS-J）的克隆形成、增殖和成熟反应，并将它们的反应与成人外周血（PB）或骨髓（BM）中祖细胞的反应进行了比较。我们发现，34周妊娠CB在不存在外源性细胞因子的情况下产生一些自发集落28 +/- 4.7 CFU-MK，并且响应于IL-3、IL-6和IL-3 + IL-6产生比其他测试样品更多的CFU-MK和BFU-MK。CFU-MK的增殖在34周时达到最大，并逐渐下降到足月。当与成人BM或PB相比时，CB衍生的CFU-MK具有增加的细胞结构，并且在用IL-3或IL-6刺激后含有显著更多经历碎片化成血小板样颗粒的细胞。照射后再生障碍性犬血清（PICS-J）是一种非常有效的刺激MK祖细胞在所有发育阶段。我们的研究结果表明，CB MK祖细胞是非常敏感的外源性细胞因子和细胞因子的增殖和成熟的反应的大小与发育年龄。
Fetal cord blood (CB) is rich in haemopoietic stem cells and progenitors. We studied the clonogenic, proliferative and maturational responses of megakaryocyte (MK) progenitors in CB, from different gestational ages, to various cytokines: IL-3, IL-6, IL-3 + IL-6, and aplastic canine serum (PICS-J), and compared their responses to those of progenitors in adult peripheral blood (PB) or bone marrow (BM). We found that 34-week gestation CB produced some spontaneous colonies 28 +/- 4.7 CFU-MK in the absence of exogenous cytokines, and produced more CFU-MK and BFU-MK in response to IL-3, IL-6 and IL-3 + IL-6 than the other samples tested. Proliferation of CFU-MK was maximal at 34 weeks and decreased gradually toward term. When compared to adult BM or PB, the CB-derived CFU-MK had increased cellularity and contained significantly more cells undergoing fragmentation into platelet-like particles after stimulation with IL-3 or IL-6. Post-irradiation aplastic canine serum (PICS-J) was a highly potent stimulator of MK progenitors at all developmental stages. Our results indicate that CB MK progenitors are exquisitely sensitive to exogenous cytokines and that the magnitude of their proliferative and maturational responses to cytokines is related to developmental age.
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影响因子：
20.3
作者：
J. Wagner;H. Broxmeyer;RL Byrd;B. Zehnbauer;B. Schmeckpeper;N. Shah;C. Griffin;P. Emanuel;K. Zuckerman;S. Cooper
通讯作者：
J. Wagner;H. Broxmeyer;RL Byrd;B. Zehnbauer;B. Schmeckpeper;N. Shah;C. Griffin;P. Emanuel;K. Zuckerman;S. Cooper
巨核细胞与内皮下细胞外基质的相互作用。
DOI：
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发表时间：
1986
期刊：
Progress in clinical and biological research
影响因子：
--
作者：
Eldor,A;Levine,RF;Caine,YG;HyAm,E;Vlodavsky,I
通讯作者：
Vlodavsky,I
白介素 3 促进分离的单个巨核细胞的分化。
影响因子：
20.3
作者：
Ishibashi,T;Burstein,SA
通讯作者：
Burstein,SA
影响因子：
158.5
作者：
GLUCKMAN, E;BROXMEYER, HE;BOYSE, EA
通讯作者：
BOYSE, EA
DOI：
10.1073/pnas.84.24.9035
发表时间：
1987-12-01
影响因子：
11.1
作者：
IKEBUCHI, K;WONG, GG;OGAWA, M
通讯作者：
OGAWA, M