Reduction of butyrate- and methane-producing microorganisms in patients with Irritable Bowel Syndrome.
Reduction of butyrate- and methane-producing microorganisms in patients with Irritable Bowel Syndrome.
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DOI：
10.1038/srep12693
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发表时间：
2015-08-04
影响因子：
4.6
通讯作者：
Manichanh C
中科院分区：
文献类型：
--
作者：
Pozuelo M;Panda S;Santiago A;Mendez S;Accarino A;Santos J;Guarner F;Azpiroz F;Manichanh C
肠易激综合征（IBS）的病理生理机制尚不清楚。在这里，我们调查了一个大型患者队列的微生物组，以确定IBS亚型的特定特征。我们检查了113名IBS患者和66名健康对照的微生物组。这些参与者的一个子集提供了两个样本，间隔一个月。我们共分析了273个粪便样本，产生了超过2000万个16 S rRNA序列。在IBS患者中，显著较低的微生物多样性与产丁酸菌的相对丰度较低相关（P = 0.002; q < 0.06），特别是在IBS-D和IBS-M患者中。与健康对照组相比，未接受任何治疗的IBS患者的甲烷菌丰度较低（P = 0.005; q = 0.05）。此外，几种细菌分类群与胀气和腹痛感之间存在显着相关性（P < 0.05）。总而言之，我们的研究结果表明，IBS-M和IBS-D患者的特征在于产丁酸细菌的减少，已知其可改善肠道屏障功能，以及产甲烷微生物的减少，这是人类结肠中氢处置的主要机制，这可以解释IBS中的腹部气体过多。
The pathophysiology of irritable bowel syndrome (IBS) remains unclear. Here we investigated the microbiome of a large cohort of patients to identify specific signatures for IBS subtypes. We examined the microbiome of 113 patients with IBS and 66 healthy controls. A subset of these participants provided two samples one month apart. We analyzed a total of 273 fecal samples, generating more than 20 million 16S rRNA sequences. In patients with IBS, a significantly lower microbial diversity was associated with a lower relative abundance of butyrate-producing bacteria (P = 0.002; q < 0.06), in particular in patients with IBS-D and IBS-M. IBS patients who did not receive any treatment harboured a lower abundance of Methanobacteria compared to healthy controls (P = 0.005; q = 0.05). Furthermore, significant correlations were observed between several bacterial taxa and sensation of flatulence and abdominal pain (P < 0.05). Altogether, our findings showed that IBS-M and IBS-D patients are characterized by a reduction of butyrate producing bacteria, known to improve intestinal barrier function, and a reduction of methane producing microorganisms a major mechanism of hydrogen disposal in the human colon, which could explain excess of abdominal gas in IBS.